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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2008年01期

Page:

75-78

Research Field:

化学

Publishing date:

Info

Title:

Optimization of Lysine 6-aminotransferase Activity by Shake-Flask Fermentation

Author(s):

Yan Yuan;  Liu Jihua;  Yu Boyang;  Zhang Jian

Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 210038, China

Keywords:

Lysine 6- am ino transferase;  optim ization;  ferm en tation;  L-PA

PACS:

TQ464.8

DOI:

-

Abstract:

The optima l fermentation cond itions for Lysine 6- am ino transferase( LAT) from Verticillium sp. in shake- flask cu ltivation we re investigated, inc luding carbon source, nitrogen source, phospha te, and surfac tant. Optim a l inorgan ic sa ltw as obta ined by orthogona l exper im ent. The resu lt show ed tha t 0.2% L-Lys, 30% Am idu lin, 0.5% KH 2 PO4, 0.1% M gSO₄ 7H ₂ O could enhance the y ie ld o f LAT. After 52h culture LAT activ ity is one time strong er than before, wh ich reached its peak, 1 217U L^{- 1.}

References:

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Last Update: 2013-05-05