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Purification of Recombinant Porcine-α Interferon and the Identification of its Biochemical Characteristics(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年02期
Page:
97-100
Research Field:
生命科学
Publishing date:

Info

Title:
Purification of Recombinant Porcine-α Interferon and the Identification of its Biochemical Characteristics
Author(s):
Shao Jing1; 2; Yu Ruisong2; 3; Dong Shijuan2; 3; Zhu Yumin2; 3; Shen Shiyuan2; 3; Cao Xiangrong1; Li Zhen2; 3
( 1. S chool of L ife S cience, Nan jing N orm alUn ivers ity, N an jing 210046, C h ina)
( 2. An im alHu sbandry and V eterinary Research Ins titu te, Shangh aiAcademy ofAgricu ltural S cien ces, Sh anghai 201106, Ch ina)
( 3. ShanghaiM un icipalKey Laboratory ofAgr-iGen et ics and Breed ing, Shanghai 201106, C hina)
Keywords:
recom binant po rc ine α- interferon( Po IFN-α);  affin ity chrom atog raphy;  ion- ex change chroma tog raphy;  glycosylation;  b io chem ica l character istics
PACS:
S852.5
DOI:
-
Abstract:
In th is study, we dev eloped the techn ique fo r pur ifying recom binant po rc ine-α inte rferon ( PoIFN-α) that w as e fficien tly expressed by P ichia Pastor is and stud ied the biochem ical character istics of PoIFN-α. Recom b inant po rcine IFN-α w as purified to homogeneity by affinity chroma tog raphy and ion-exchange chrom atog raphy stepw isely afte r cen trifuga tion、dialysis and filtration. The resu lts of its b iochem ica l characteristics show ed that the first five am ino acids of Nterm inal o f the purified pro tein w as correct. The pro tein w as heat and ac id- stab le and the dithio la te-bond was cruc ia l to the pro te in activ ity. No g lycosy la tion w ere found on the target pro te in

References:

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Last Update: 2013-05-05