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Effect of Hesperetin on Release of Nitric Oxide From Endothelial Cells(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年03期
Page:
96-99
Research Field:
生命科学
Publishing date:

Info

Title:
Effect of Hesperetin on Release of Nitric Oxide From Endothelial Cells
Author(s):
Xu Dongmei; Liu Li; Cheng Yiyu
Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310058,China
Keywords:
hesperetin;  hum an um bilical ve in endo the lial ce lls ( HUVEC s);  nitr ic ox ide;  estrogen receptor
PACS:
R285
DOI:
-
Abstract:
Ob jective to investigate the e ffect o f hesperetin on re lease of n itr ic ox ide from cu ltured hum an um bilical ve in endothelial cells ( HUVECs) and poss ible m echan ism. M e thods F luo rom etr ic assay w ith 2, 3-diam inonaphthalene ( DAN) w as em ployed to determ inate the concentration of n itr ite in m ed ium, indica ting NO production from HUVECs. Them odifiedMCF- 7 cell pro liferation assay and the reporter-gene assayw ere used to eva luate the estrogenic activ ity o f hespere tin. Results at re la tive low leve l o f endogenous estrog en, hespe retin ( 12.5~ 100 μmo l/L) prom o ted the release o f NO from HUVECs in a dose-dependent m anner, wh ich cou ld be inhib ited by IC I182, 780 and actinom yc in D. W hen HUVECs w ere treated w ith comb ination o f hesperetin and E2, the leve l of NO re leased from HUVEC sw as low er than that o f sing le treatment. Under h igh level of endogenous estrog en, hesperetin inhib ited the release of NO from HUVECs. H espe re tin sign ifican tly promo ted the pro liferation ofMCF- 7 ce lls, wh ich cou ld be comp le tely b locked by ICI182, 780. M eanwh ile, hespere tin appeared to com prom ise e ffic iency o f E2. A lso hesperetin activa ted estrogen receptor and induced the expression of Lucife rase ( reporter gene). Conclusion hesperetin can be classified as a partia l agonist of estrog en-receptor and possess regulation activ ity on NO production from HUVEC s. The possible m echan ism invo lves in estrogen ic ac tiv ity and regu lation o f gene expression

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Last Update: 2013-05-05