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Effect of Lutein on the Differentiation of Osteoclast Induced From Bone Marrow in Mice(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年03期
Page:
100-103
Research Field:
生命科学
Publishing date:

Info

Title:
Effect of Lutein on the Differentiation of Osteoclast Induced From Bone Marrow in Mice
Author(s):
Pei Lingpeng; Cui Jian
Central University for Nationalities,Research Center of Minarity Traditional Medicine,Beijing 100081,China
Keywords:
lutein;  osteoc last;  differentiation
PACS:
Q25
DOI:
-
Abstract:
Effect o f lute in on the d iffe rentia tion of osteo clast induced from bonem arrow were studied in v itro. M ononuclea r ce lls o f m ice bonem arrow w ere incubated w ith DMEM conta in ing macrophage co lony stimu la ting fac to r (M - CSF) and recepto r activato r of NF - kB lig and ( RANKL). The cu lture was treated w ith lute in of d ifferent concentrations ( 10- 8 m ol/L、10- 7 m o l/L、10- 6 mo l/L) , respective ly. The cu lture ce lls w ere fixed and w ere stained fo r tarta te-resistant ac id ic phospha tase ( TRAP) a fter 7d. The form ation of osteoc lasts w as quantified by counting the num be r of TRAP+ mu ltinuc lea r ce lls. The percentage of bone resorbed surface, TRAP activ ity and the expression o f receptor ac tivator o f NF- kB ( RANK) mRNA w ere ana ly sed. The appearance o f reduced m ice osteo clasts w as typica.l Com pared w ith the contro l g roup, the num be r of TRAP+ mu ltinuc lea r cells, TRAP activity and the percentage of bone surface in lute in m idd le-do se group and lu tein h igh-dose g roup sign ificantly decreased( P < 0.05) . But the expression o f RANK mRNA sign ifican tly increased( P < 0.05 ) by treated in different doses o f lute in. Lute in can inh ib it o steoc lasts d ifferentiation in v itro.

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Last Update: 2013-05-05