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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2008年03期

Page:

104-108

Research Field:

生命科学

Publishing date:

Info

Title:

Isolation and Identification of a Photosynthetic Bacteria Producing Coenzyme Q₁₀

Author(s):

Li Xun; Zhang Jinglei

School of Chemistry Engineering,Nanjing Forestry University,Nanjing 210037,China

Keywords:

photosynthetic bacter ia;  iso lation;  stra in iden tification;  16S rDNA

PACS:

X172

DOI:

-

Abstract:

The iso lates produc ing C oenzyme Q_l0 ( CoQ10 ) of purp le non sulfur photosynthetic bac teriaw ere enriched out o f pond sludge, one iso late nam ed LZC w as se lec ted based on its h igh CoQ_l0 content and identified system atically. The stra in LZC g rows anaerob ica lly in the light and ae robically in the dark. Optim al grow th occurs at 30 ~ 35℃ and at pH 7.0~ 8. 0, Various o rganic compounds are used as photosynthe tic e lectron donors and carbon sources. The CoQ₁₀w as ex tracted from the sam ple w ith organ ic so lvent by ultrason ic extraction and determ ined by the UV m ethod. The w ay, cheap and faster, w as the proper se lectw ay of PSB strain w ith h igh CoQ₁₀ concentration. The CoQ10 concentra tion determ ined by UV way o f stra in c reached 9.49μg /mL. A phy logene tic ana lys is based on 16S rDNA gene sequences revea ls tha t strain LZC gathers a cluster w ith 4 stra ins of R hodobacter sp. w hose access ion number in GenBank are AB251407. 1, AB251408.1, AB017799.1, DQ342322. 1, respective ly. The stra in LZC subcultures stab ly at least 15 genera tions. The results presented here dem onstrated strain LZC is Rhodobacter blasticus.

References:

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Memo

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Common functions

Last Update: 2013-05-05