|Table of Contents|

Comparison of Gradient Polyacrylamide and Polyacrylamide Gel Electrophoresis in the Isolation and Identification of Plant SOD Isozyme(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2008年04期
Page:
97-103
Research Field:
生命科学
Publishing date:

Info

Title:
Comparison of Gradient Polyacrylamide and Polyacrylamide Gel Electrophoresis in the Isolation and Identification of Plant SOD Isozyme
Author(s):
Cheng Guangyu1; Wang Feng2; Tao Minxuan2; Lu Changmei1; Wu Gongrong1; Chang Fuchen1
1.School of Life Science,Nanjing Normal University,Nanjing 210097,China
2. Jin l ing C ollege, N an jing Norm alUn iversity, Nan jing 210097, Ch ina
Keywords:
GPGE;  PAGE;  SOD;  Iso lation;  Identifica tion
PACS:
Q946
DOI:
-
Abstract:
Th is paper compared the app lication of grad ient po ly acry lam ide ge l e lectrophresis( GPGE) and po lyacrylam ide ge l e lectrophoresis( PAGE) in iso lation and identifica tion o f plant SOD iso zym e. The resu lts show ed that SOD bands on GPGE w ere div ided into three reg ion of A, B, C accord ing to itsRf. TheRf o fA reg ion SOD band was m inim um, it w as sensitive to 1% SDS and w asM n-SOD. The Rf o f C reg ion SOD band w as the biggest, it was no t sensitive to 1% SDS and w as CuZn-SOD. The Rf of B reg ion SOD band was betw een A and C, it was sensitiv e to 1% SDS and was Fe-SOD. The three SOD iso zym es w ere d istinguished by its Rf and sensitiv ity to SDS on polyacry lam ide g radient ge,l th is resu lt w as about the sam e as the resu lt of judgem en t using inh ibitor. The sam e plant sam plew as iso lated by PAGE d id not have the charac teristic. 10 - 13 m o l and 10 - 15 m ol bov ine SOD w as detected in PAGE and GPGE, respective ly. Four and tw o SOD activ ity bands w ere detected in GPGE and PAGE at 1.56 ×10 - 12 m o l bov ine SOD, respectiv ely. TheRf of Fe-SOD from Lycium chinense w as the bigg est in 7. 5% PAGE and it ove rlapped w ith CuZn-SOD in 10% PAGE. The FeSOD and M n-SOD o f cirus w ilsonii, Mn-SOD and CuZn-SOD of C itrus ery throsa w ere ove rlapped each o ther in 10% PAGE, but three types o f SOD w ere com plete ly iso la ted in 4% ~ 35% GPGE. The re liab ility o f iso lating and identify SOD using GPGE w as expounded by tw o tim es g el concentration grad ient electrophores is TGCGE, the Rf and the num be r of SOD bands in GPGE w ere com patible w ith TGCGE, and it can com plete ly iso late and identify the overlapped three SOD types on PAGE.

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Last Update: 2013-05-05