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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2008年04期

Page:

124-128

Research Field:

生命科学

Publishing date:

Info

Title:

Expression and Monoclonal Antibody Preparation of 4Ig Fragment of Long Myosin Light Chain Kinase

Author(s):

Lü Ning¹; ²; Zhu Chunhua¹; Zhang Wencheng²; Zhu Minsheng²; Chen Huaqun¹

1.The Key Laboratory of Molecular and Medical Biotechnology of Jiangsu Province,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
2. M odelAn im alResearch C enter, Nan jing Un ivers ity, Nan jing 210061, Ch in a

Keywords:

L- MLCK;  MLCK 4 Ig;  prote in expression;  monoclona l an tibody

PACS:

Q78

DOI:

-

Abstract:

The functions of longm yosin light cha in k inase (MLCK) and the m ultip le dom a ins w ith in the m olecule were poorly understood. To our know ledge, there is no specific antibody aga inst L- MLCK so far, wh ich is essen tia l for the study o fL- MLCK. In this report, the 4 Ig fragm ent o f ch icken L- MLCK (MLCK4Ig ) w as expressed by using ofE. co li BL21 system, the pur ified prote in was used as antigen to induce antibody. Spec ific antibody was detected in the sera o f m ouse in jected w ith chickenMLCK4Ig. W e further estab lished two hybr idom a ce ll lines produc ingm onoc lonal an tibody, wh ich specifically reac ted w ith ch ickenMLCK4Ig o r ch icken L- MLCK. The character istic and usefulness o f the ant-i body we re a lso discussed. The resu lts show ed that ourwo rk partially so lved the prob lem o f L- MLCK antibody de fic iency, w hich prov ides an im portant too l fo r the functional investigation of L-M LCK andM LCK4Ig fragm ent

References:

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Memo

Memo:

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Common functions

Last Update: 2013-05-05