|Table of Contents|

Effect of Manganese on Reactive Oxygen Species Metabolism and Submicroscopic Structure of Potamogeton crispus(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年01期
Page:
105-109
Research Field:
生物学
Publishing date:

Info

Title:
Effect of Manganese on Reactive Oxygen Species Metabolism and Submicroscopic Structure of Potamogeton crispus
Author(s):
Xu Xiaoying; Shi Guoxin; Xu Qinsong; Yang Haiyan; Xu Ye; Ding Chunxia
School of Life Science,Nanjing Normal University,Nanjing 210046,China
Keywords:
P otam ogeton crisp us;  M n 2+ ;  stress;  subm icroscopic structure;  ROS
PACS:
Q945
DOI:
-
Abstract:
The tox ic effect of various concentrations ofMn 2+ ( 0、0. 09、0. 18、0. 27、0. 36mmo l/L) w as stud ied on antiox-i dant system, active oxygen generation and subm icro scop ic structu re of Po tam og eton crisp us. The results show ed that the content of chlorophy ll a, ch lo rophyll b and to tal ch lorophyll a ll first increased and then decreased w ith increas ing the concentration o fM n 2+  . Superox ide d ism utase ( SOD ) activ ity g enerally increased, peroxydase ( POD ) and catalase ( CAT) ac tiv ities enhanced a several fo ld, but w ith the m anganese concentration increased continuously, they a ll dec reased, wh ich w as po ssib ly the reason o f accumu lation o f O- 2 and H2O 2. G lutathione ( GSH ) and antiscorb ic ac id ( A sA) conten ts changed at the sam e trend o f POD and CAT. The leve l o f lipid perox idation w asm easured as the content o fm a londialdehyde (MDA), w hich w as a lw ays increased and w as the same as the change o f O- 2 and H  2O 2. Transm iss ion electron m icroscopy observation indicates that the h igherM n2+ concentration, the mo re serious dam ages to the leaf ce lls, especia lly ch lo roplast, m itochondrion and nucleus. The conclus ion could be reached tha t the grow th declin ing even death of plantw as resu lted from destruc tion o f structure foundation of physiolog ica l function, interruption o f ion and enzym es equilibr ium and disorde r o f an tiox idant system. But the influences of those aspects a re in different ex ten t in Po tam ogeton crispus.

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Last Update: 2013-04-23