|Table of Contents|

Development and Potential Application of a Nested PCR for the Detection of White Spot Syndrome Virus in Procambarus Clarkia(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年02期
Page:
98-102
Research Field:
生命科学
Publishing date:

Info

Title:
Development and Potential Application of a Nested PCR for the Detection of White Spot Syndrome Virus in Procambarus Clarkia
Author(s):
Li Wenjie; Zhou Guoqin; Zhu Feili; Wang Weidong; Zhou Guoping
Nanjing Center for Control and Prevention of Aquatic Animal Infections Disease,Nanjing Aquatic Sciences Institute,Nanjing 210036,China
Keywords:
wh ite spot syndrom e v irus;  nested PCR;  Procam barus c la rkii
PACS:
S945.4
DOI:
-
Abstract:
Four specific pr im er, W1, W2, S1 and S2, we re des igned according to theVP28 gene sequences o f 3 w hite spo t syndrom e v irus (WSSV ) strains depo sited in the GenBank. PCR de tecting the VP28 gene ofW SSV from Procam ba rus clark ii was estab lished after se lection o f the optim al reaction cond itions. Sensitiv ity of the first and second amp lifications by the nested PCR w as 36 ng and 36 pg, respectively. Am ong 9 c lin ical Procam barus c la rkii sam ples to be tested by nested PCR, therew as no positive case detec ted by the first PCR amp lifications w ith pr im ersW1 andW2, and oneW SSV positive case detec ted by the second PCR amp lificationsw ith prim ers S1 and S2. The results showed that the nested PCR established by th is study w as much mo re su itab le than the one step PCR for the detection ofWSSV in Procambarus clark ii.

References:

[ 1] 蔡生力, 黄倢, 王崇明, 等. 1993~ 1994年对虾暴发病的流行病学研究[ J]. 水产学报, 1995, 13( 2): 112-117.
[ 2] InouyeK, M iwa S, OsekoN, e t a .l M ass mo rtalities o f cultured kurum a shrim p Penaeus japonicus in Japan in 1993: e lectronm ic roscopic ev idence of the causative v irus [ J] . Fish Pa tho logy, 1994, 29( 2): 149-158.
[ 3] Ligh tnerD V, Redman R M, Pou lo s B T, et a.l R isk of spread o f penae id shr imp v iruses in theAm ericas by the inte rnationalm ovem ent of live and frozen shrim p[ J]. Rev Sc iTech, 1997, 16( 1): 146-60.
[ 4] 朱建中, 陆承平. 对虾白斑综合征病毒在鳌虾动物模型的感染特性[ J]. 水产学报, 2001, 10( 1): 47-51.
[ 5] 朱建中, 陆承平. 用动物模型检验消毒剂对对虾白斑综合征病毒的灭活效果[ J]. 中国兽药杂志, 2001, 35( 2): 6-7.
[ 6] Ve terinary Serv ices Centers for Epidem io logy and An im alH ea lth. CEI Im pactW orksheetW hite Spot D isease Lou isiana, USA
[ OL /R]. [ 2007-03-16]. http: / /www. aphis. usda. gov /vs /ceah / cei/ taf/ iw- 2007- files /WSDUS- LA2007 /w sd- us- la- 05-17- 07. pd.f
[ 7] 黄捷, 宋晓玲, 于佳, 等. 杆状病毒性的皮下及造血组织坏死——对虾暴发性流行病的病原和病理学[ J]. 海洋水产研究, 1995, 16( 1) : 1-10.
[ 8] 涂小林, 钟江, 高双诚, 等. 中国对虾一种杆状病毒的ELISA 检测方法[ J]. 水产学报, 1995, 19( 4): 315-321.
[ 9] 吕玲, 何建国, 邓敏, 等. 核酸探针原位杂交检测白斑综合症病毒的组织特异性[ J]. 热带海洋, 2000, 19( 4): 86-91.
[ 10] Otta S K, Karunasagar I. Detection of m onodon bacu lov irus and white spo t syndrom e v irus in apparently healthy Penaeus
m onodon po stlarvae from Ind ia by po lym erase cha in reaction[ J]. Aquacu lture, 2003, 220( 1/4): 59-67.
[ 11] 谢树涛, 何建国, 杨晓明, 等. 套式PCR 检测斑节对虾白斑症病毒(WSSV ) [ J]. 青岛海洋大学学报, 2001, 31( 3):220-224.
[ 12] H ossa inM S, Chakraborty A, Joseph B, et a.l Detection of new hosts forw hite spot syndrom e v irus of shr im p using nested
po lym erase cha in reaction[ J]. Aquaculture, 2001, 198( 1 /2): 1-11.
[ 13] 庞耀珊, 谢芝勋, 谢志勤, 等. 二温式PCR 检测对虾白斑综合征病毒[ J]. 中国兽医杂志, 2003, 39( 4): 43-45.
[ 14] 朱建中, 夏晓勤, 陆承平, 等. 斑点杂交检测对虾白斑综合征病毒青岛株在螯虾体内的动态分布[ J]. 中国病毒学,2001, 16( 1): 92-95.

Memo

Memo:
-
Last Update: 2013-04-23