|Table of Contents|

Studies on Condition Protocorm Differentiation and Root Induction of Dendrobium officinale by Orthogonal Design(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2009年04期
Page:
98-102
Research Field:
生命科学
Publishing date:

Info

Title:
Studies on Condition Protocorm Differentiation and Root Induction of Dendrobium officinale by Orthogonal Design
Author(s):
Shao Shiguang1; 2; Hou Beiwei1; Zhou Qi1; Zhang Xiaoxiao1; Lu Beibei1; Bai Weiya1; Ding Xiaoyu1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
Keywords:
Dendrobium officinale;  tissue cu lture;  protocorm d ifferentiation;  roo t induction;  orthogonal expe rim en t
PACS:
S567.239
DOI:
-
Abstract:
To study the effects of d ifferent fac to rs on pro tocorm differentiation and roo t induction o fDendrobium off icinale, tw o g roups of three kinds of facto rs on three levels we re used by o rthogona l des ign. The dataw ere analyzed w ith rang e ana ly sis and va riance analysis. Resu lts show ed that the optim alm edia to induce cluster shee t from protocorm w as N6 medium w ith 6-BA 2m g /L and IBA 0.2m g /L and the pH 5. 8. The effic iency o f the three facto rs w as in sequence of IBA > 6-BA > pH. On the o ther hand, N6 m edium w ith KC l 2.5 m ol/L and agar 4 g /L was su itab le for roo t induction o f Dendrobium officinale. Them ain factorwh ich influenced roo t induc tion was KC l concen tration, and the o the rs m ino r factors w ere ag ar concentra tion and type of basicm edium.

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Last Update: 2013-04-23