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Molecular Cloning and Characterization of a Novel Gene,EJO3,Putatively Involved in the Ovarian Development of Chinese Mitten Crab(Eriocheir Japonica Sinensis)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年02期
Page:
71-76
Research Field:
生命科学
Publishing date:

Info

Title:
Molecular Cloning and Characterization of a Novel Gene,EJO3,Putatively Involved in the Ovarian Development of Chinese Mitten Crab(Eriocheir Japonica Sinensis)
Author(s):
Ma Changyan; Guo Yujie; Zhou Kaiya
Jiangsu Key Lab for Biodiversity and Biotechnology,School of Life Science,Nanjing Normal University,Nanjing 210097,China
Keywords:
suppression subtractive hybr idiza tion ( SSH );  cDNA m acroa rray;  Er io cheir japonica sinensis;  ovary;  EJO 3
PACS:
S966.16
DOI:
-
Abstract:
In the present study, w e pe rfo rm ed suppression subtractive hybr id ization ( SSH ) comb ining cDNA m acro array to identify genes putative ly invo lved in the ovar ian dev elopment of Ch inese m itten crab. A nove l g ene, EJO3 ( GenBank accession number: AY185919) w hose expression is h ighe r at stage III than that at stage II w as c loned by 5‘and 3’ RACE. The ORF ( open reading fram e, ORF) o f the cDNA o fEJO 3 consisted of 1 368 bp encod ing 455 am ino acids. The pI/Mw deduced from the am ino ac ids sequence w as 5. 79 /50 000. Pro te in coded byEJO 3 genew as probab ly a sec retion pro te in w ith aVWD dom a in. The d ifferen tia l express ion o fEJO3 w as further confirm ed byNo rthern blot ana lys is. Express ion profile ana ly sis show ed that EJO3 is h ighly expressed in ova ry and the re is little or no express ion o fEJO3 in intestine, heart, m usc le and hepatopancreas. Results of this study have made an important foundation to further eluc-i date them o lecu lar m echan ism o f the ova rian deve lopm ent of Ch inese m itten crab.

References:

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Last Update: 2013-04-08