|Table of Contents|

Screening and Analysis of the Differential Expressed Sequence of Diamondback Moth,Plutella xylostella(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年02期
Page:
91-95
Research Field:
生命科学
Publishing date:

Info

Title:
Screening and Analysis of the Differential Expressed Sequence of Diamondback Moth,Plutella xylostella
Author(s):
Liu Baiduo1; Li Fengliang2; He Bilei1; Yu Xizhong1; Li Zhongying2; Cheng Luogen1
1.School of Life Science,Nanjing Normal University,Nanjing 210046,China 2. Institute of Plant P rotect ion, Gu izhou A cadem y of Agricu ltu re Sciences, Gu iyang 550006, China
Keywords:
Plutella X y lostella ( L. );  inher itance of res istance;  co lony PCR;  cDNA RDA
PACS:
S433
DOI:
-
Abstract:
W e sifted the d ifferential expressed sequence from P lutella xy los tella susceptible stra in and the deltam ethrinres istance stra in by tw o-w ay cDNA representa tiona l d ifference ana ly sis( cDNA RDA). The tw o-way cDNA RDA was perform ed respective ly using cDNA from the P. xy loste lla susceptib le stra in as Tester amp licon and from the de ltame thr in-res istance stra in as Dr iver am plicon, and us ing cDNA from the deltam ethrin-resistance stra in as Tester am plicon and from the susceptib le stra in as Dr iver amp licon. The differential sequences be tw een P lutella xy los tella susceptib le stra in and the de ltam ethrin- resistance stra in w ere analysed prelim ina rily. A fter three rounds o f subtractive hybr idiza tion, the d ifferentia l fragm entsw ere cloned w ith pMD18-T vector, then screen ing positive clone w ith amp ic illin and sequenced a fter co-l ony PCR. Compar isons between our sequencing result and da ta in GenB ank show ed that therew ere two sequences appearing re la tive ly high hom ology to some know n sequences. One o f them had h igh hom o logy w ith the sequence encoding s3a prote in.

References:

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Last Update: 2013-04-08