|Table of Contents|

Screening the Differentially Expressed Genes Related to the Ovarian Development of Chinese Mitten Crab(Eriocheir japonica sinensis) Using cDNA Macroarray(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年03期
Page:
76-80
Research Field:
生命科学
Publishing date:

Info

Title:
Screening the Differentially Expressed Genes Related to the Ovarian Development of Chinese Mitten Crab(Eriocheir japonica sinensis) Using cDNA Macroarray
Author(s):
Ma Changyan 1; 2; Guo Yujie 1; Zhou Kaiya 1
1.Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China 2. Department of Developm ental Genetics, Nan jing M ed icalU n ivers ity, N an jing 210029, Ch ina
Keywords:
E riocheir jap onica s inensis;  ovary;  suppression subtractiv e hybr id ization ( SSH );  cDNA macroarray;  expressed sequence tag
PACS:
S917.4
DOI:
-
Abstract:
To c lone genes w hich a re re lated to the ovar ian deve lopm ent of Chinesem itten crab, the sub trac ted libra ries have prev iously been construc ted. In the present study, the cDNA m acroarray was prepared to screen the diffe rentia lly expressed g enes and fifty clones identified from the fo rw ard subtrac ted cDNA libra ry w ere selected random ly and sequenced. In tota ,l 167 c lones whose signa l d iffe rence w as > 2 fo lds we re obta ined. O f the d ifferentially expressed clones, 104 c lones w ere h ighly expressed in the ovary at stageⅢ  , 63 c lones w ere h ighly expressed in the ovary a t stage Ⅱ . M oreover, seven independent ESTs we re obta ined from the fo rw ard sub trac ted cDNA library. H om o logy analysis showed that there aren t’ sequences significantlym atching to these ESTs. So theyw ere deduced to be nove l ESTs, and a ll o f them were deposited in dbEST. The accession num be rs are: CA591892、CA591893、CA591894、CA591895、 CA591896、CA591897 and CA591898, respec tive ly.

References:

[ 1]L iH D, Yu GM, Fan JR, e t a .l Experim ents on theCu lture o fYoungM itten Crab ( Eriocheir sinensis) [M ]. Be ijing: Ch-i na Ocean Press, 1991, 93-98.
[ 2]M u Y Y, Shim K F, Guo J Y. Effects o f prote in level in iso ca lo ric d ie ts on grow th per fo rm ance o f the juven ile Chinese ha iry crab, Er iocheir sinensis [ J]. Aquaculture, 1998, 165: 139-148.
[ 3]W angW, Zhu N, Gu Z, et a.l Study on the transm ission o f trem or d isease in the Ch inese m itten crab, Er iocheir sinensis ( C rustacea Decapoda) [ J]. J Inve rtebr Patho ,l 1998, 81: 202-204.
[ 4]Jin G, X ie P, L i Z. Food hab its o f 2-year-o ld Chinese m itten crab, E riocheir sinensis, stocked in Lake B aoan [ J]. J o f Freshw ater Eco logy, 2003, 18( 3): 369-375.
[ 5]Tang B, Zhou K, Song D, et a.l M o lecu la r system atics of the Asian m itten crabs, genus E rioche ir ( Crustacea: B rachyura) [ J]. Mo l Phy logenet Evo ,l 2003, 29( 2): 309-316.
[ 6]Sun H, Zhou K, Song D. M itochondr ia l genom e o f the Ch inesem itten crab E riocheir japonica sinenesis ( Brachyura: Thoracotrema ta: Grapso idea) reveals a nove l gene o rder and two targ et reg ions o f gene rearrangem ents [ J]. Gene, 2005, 349: 207- 217.
[ 7]W ang C, Li C, Li S. M itochondr ia l DNA- in ferred population structure and dem og raph ic h istory o f them itten crab ( E riocheir sensu stricto ) found along the coast of m ainland China [ J]. M ol E co ,l 2008, 17( 15): 3 515-3 527.
[ 8]Diatchenko L, Lau Y F, Cam pbellA P, et a.l Suppression subtractive hybr id ization: am e thod for genera ting d iffe rentia lly regu la ted or tissue-spec ific cDNA probes and libraries[ J]. ProcN atlA cad Sc iUSA, 1996, 93: 6 025-6 030.
[ 9]马长艳, 周开亚, 郭豫杰, 等. 中华绒螯蟹卵巢差减cDNA 文库的构建[ J]. 动物学研究, 2003, 24( 1): 53-56.
[ 10]Yang G P, Ross D T, KuangW W, e t a .l Comb ining SSH and cDNA m icro arrays fo r rapid identifica tion of differentially expressed genes [ J]. Nucle icAc ids Res, 1999, 27( 6): 1 517-1 523.
[ 11]Vo iblet C, Dupless is S, Encelot N, e t a.l Identifica tion o f sym biosis- regulated genes in Eucalyptus g lobulus-Piso lithus tinctor ius ec to-m ycorrh iza by differential hybr idiza tion o f arrayed cDNAs [ J]. Plan t J, 2001, 25( 2): 181-191.
[ 12]Porkka K P, V isako rpi T. Detection o f d ifferen tia lly expressed genes in prostate cancer by com bin ing suppress ion sub tractive hybridization and cDNA library array [ J]. J Patho,l 2001, 193( 1): 73-79.
[ 13]L iX D, Essayan D M, L iuM C, et a .l Pro filing o f differential gene expression in activa ted, alle rgen- spec ific hum an Th2 ce lls [ J]. Genes Imm un, 2001, 2( 2) : 88-98.
[ 14]Gardm o C, Sw erdlow H, M ode A. G row th horm one regulation of rat liver g ene expression assessed by SSH and m icroa rray [ J]. Mo l Ce llEndocr ino,l 2002, 190( 1-2): 125-133.
[ 15]H adjia rgyrouM, Lombardo F, Zhao S, et a.l T ranscr iptional profiling of bone regene ration. Insight into them o lecular complex ity o f wound repair [ J]. J B io l Chem, 2002, 277( 33): 30 177-30 182.

Memo

Memo:
-
Last Update: 2013-04-08