|Table of Contents|

Molecular Cloning and Expression of the Bile Salt Hydrolase Gene(bsh) From Lactobacillus plantarum Y1(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2010年03期
Page:
91-96
Research Field:
生命科学
Publishing date:

Info

Title:
Molecular Cloning and Expression of the Bile Salt Hydrolase Gene(bsh) From Lactobacillus plantarum Y1
Author(s):
Huang QianHuang LuPan DaodongYang Yao
Branch Center of National Dairy Products Processing Technology Development,Department of Food Science and Nutrition,Nanjing Normal University,Nanjing 210097,China
Keywords:
Lactobacillus p lantarum B ile sa lt hydro lase prokaryo tic expression fus ion tags techno logy
PACS:
Q78
DOI:
-
Abstract:
Based on the hom o logous prote in BLAST result, the bsh gene o fLactobacillus p lantarum Y1( 975 bp) was amp lified by PCR and subcloned in to expression vecto r pET-28a. The constructed recomb inant plasm id pET-28a-BSH w as transform ed toE scherichia coli BL21( DE3) fo r express ion under induc tion o f IPTG. SDS-PAGE pro file ind icated that the expressed prote in ma in ly ex isted in a form of inclusion body. The so lubility of the expressed prote in w as not improved when choo sing E. coli Rose tta ( DE3) as the expression host that cou ld supply additional tRNA for rare codons. H ow eve r, w hen pLS-IF2 was chosen to construct the expression p lasm id that conta ined the fusion pro te in tag  IF2, SDSPAGE profile andW estern b lo t results showed that the expressed product IF2-BSH junction to improve the so lub ility eff-i c iency, respectively. The re fo re, on basis of the fusion tags techno logy, the b sh gene ofL. p lan tarum Y1 was successfu-l ly c loned and expressed in E. coli. , wh ich la id a foundation of study on b ioactiv ity and re lation betw een struc ture and functions o f B ile Sa ltH ydrolase from Lactobacillu s sp.

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Last Update: 2013-04-08