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Methylation of NRAGE Regulates Its Expression in the Breast Cancer Cell Lines(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2011年04期
Page:
89-95
Research Field:
生命科学
Publishing date:

Info

Title:
Methylation of NRAGE Regulates Its Expression in the Breast Cancer Cell Lines
Author(s):
Huang Yinhua; Huang Xiulun; Wen Chuanjun
Institute of Molecular Cell Biology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
Keywords:
NRAGE gene; gene regulation; DNA methylation
PACS:
R737.9
DOI:
-
Abstract:
Objective: To detect the methylation status of the 5pCpG island located in the promoter region of hNRAGE gene and its regulation of the expressions of NRAGE in the breast cancer cells． Methods: The Real-time PCR method was used to detect the mRNA levels of NRAGE gene in the breast cancer cell lines of MDA-MB-231 and MCF-7． The existence of CpG island of hNRAGE gene was analysed by the Methyl Primer Express Software( Applied Biosystems) ． Human breast cancer cell lines of MDA-MB-231 and MCF-7 were treated with 5-Aza-CdR． The expression of NRAGE was then detected by the semi-quantitative polymerase chain reaction ( RT-PCR) and Westen blot analysis． The methylation of NRAGE gene in the two kinds of cell lines was detected by the methylation-specific polymerase chain reaction ( MSP) ． Results: CpG island existed in the promoter region of NRAGE gene． The CpG island of NRAGE was methylated in MDA-MB-231 cells and unmethylated in MCF-7 cells． The expression of mRNA level in MDA-MB-231 cells was higher than in MCF-7 cells． The RT-PCR and Westen blot analysis showed that NRAGE expression was increased significantly in MDA-MB-231 cells but remained the same in MCF-7 cells after exposuring to the demethylating agent 5-Aza- CdR． Conclusion: The methylation status of NRAGE gene can regulate its expression in the breast cancer cell lines．

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Last Update: 2013-03-21