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Recombineering Mediated Chromosal Gene Knockout of Pseudomonas Putida KT2440(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2011年04期
Page:
96-101
Research Field:
生命科学
Publishing date:

Info

Title:
Recombineering Mediated Chromosal Gene Knockout of Pseudomonas Putida KT2440
Author(s):
Yang Yunwen; Jiang Fuhuan; Song Jie; Shang Guangdong
Jiangsu Key Laboratory for Microbes and Functional Genomics,School of Life Sciences, Nanjing Normal University,Nanjing 210046,China
Keywords:
Pseudomonas putida KT2440; gene knockout; recombineering
PACS:
Q78
DOI:
-
Abstract:
Pseudomonas putida KT2440 plays an important role in the degradation of environmental organic compounds and it is a good host for foreign gene expression． Gene knockout is essential to the investigations of genes and proteins． Classic chromosal gene knockout methods usually involve the gene cloning steps，which are often tedious，time consuming and tend to introduce mutations． In this paper，a recombineering strategy characterized by the homologous recombination between the PCR product and the genome，is used for the P． putida KT2440 gene knockout． This strategy is highly efficient，convenient，rapid and amendable for the simultaneously manipulation of many genes，and can be used as a general gene knockout method．

References:

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Last Update: 2013-03-21