|Table of Contents|

Construction of Recombinant pDsRed-LC3 Fusion Protein and Its Expression and Localization in HepG2 Cell(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2012年01期
Page:
84-88
Research Field:
生命科学
Publishing date:

Info

Title:
Construction of Recombinant pDsRed-LC3 Fusion Protein and Its Expression and Localization in HepG2 Cell
Author(s):
Peng WanxinSun YaoxiangChen ChenJin JieShao GenbaoWang Lei
School of Medical Sciences and Laboratory Medicine,Jiangsu University,Zhenjiang 212013,China
Keywords:
red fluorescent proteinMAP-LC3autophagyhepatocarcinoma
PACS:
R735.7
DOI:
-
Abstract:
In this study,according to the nucleotide sequence of MAP-LC3,we successfully constructed the vector that expressed the fusion protein of MAP-LC3 and red fluorescent protein ( RFP) in mammalian cells. The recombinant plasmid was identified by restriction endonuclease enzyme analysis and DNA sequence analysis. The plasmid was transfected into HepG2 cells,and the expression and distribution of the MAP-LC3 were observed with Confocal Laser Scanning Microscopy ( CLSM) after 48 hours. All of the results indicated that this vector was highly expressed in the HepG2 cells. The red fluorescence was found over the cytoplasm by CLSM. Therefore,we had a conclusion that a recombinant plasmid of MAP-LC3 and RFP was constructed successfully,and it would be an important and convenient tool to study the process of autophagy in HCC.

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Last Update: 2013-03-11