|Table of Contents|

Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene)/Carbon Nanotubes Composite Film Modified Electrode and Its Application to Determination of 8-Hydroxy-2′-Deoxyguanosine(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2012年02期
Page:
66-71
Research Field:
化学
Publishing date:

Info

Title:
Electrochemical Preparation of Poly(3,4-Ethylenedioxythiophene)/Carbon Nanotubes Composite Film Modified Electrode and Its Application to Determination of 8-Hydroxy-2′-Deoxyguanosine
Author(s):
Pan Jichao; Yao Fei; Ren Tengfei; Jia Liping; Jia Wenli; Cui Hui; Wang Huaisheng
College of Chemistry and Chemical Engineering,Liaocheng University,Liaocheng 252059,China
Keywords:
8-Hydroxy-2’-deoxyguanosine; poly( 3; 4 -ethylenedioxythiophene) ; carbon nanotubes; conductive polymer;  uric acid
PACS:
O657.1
DOI:
-
Abstract:
The poly( 3，4-ethylenedioxythiophene) /carbon nanotubes ( CNTs) composite film was prepared on the glassy carbon electrode surface by one-step galvanostatic deposition and used for the study of the electrochemical behavior of 8- hydroxy-2’-deoxyguanosine ( 8-OH-dG) ． The experimental results showed that the hybrid film can combine the advantages of conductivity of PEDOT and the unique electronic performance of CNTs and effectively enhance the adsorption and electrochemical oxidation of 8-OH-dG． Moreover，this proposed hybrid film modified electrode also showed excellent reproducibility and stability in the experiment． In 0. 1 mol /L pH 9. 0 phosphate buffer solution，the oxidative peak currents of 8-OH-dG had two good linear relationships with its concentration in 0. 014 － 14μmol /L and 14 － 56 μmol /L，with the detection limit of 35 nmol /L ( S /N = 3) ．

References:

［1］ Kasai H． Analysis of a form of oxidative DNA damage，8-hydroxy-2’-deoxyguanosine，as a marker of cellular oxidative stress during carcinogenesis［J］． Mutat Res，1997，387( 3) : 147-163．
［2］ Toraason M． 8-Hydroxydeoxyguanosine as a biomarker of workplace exposures［J］． Biomarkers，1999，4( 1) : 3-26．
［3］ Li C S，Wu K Y，Chang-Chien G P，et al． Analysis of oxidative DNA damage 8-hydroxy-2’-deoxyguanosine as a biomarker of exposures to persistent pollutants for marine mammals［J］． Environ Sci Technol，2005，39( 8) : 2 455-2 460．
［4］ Shigenaga M A，Ames B N． Assay for 8-hydroxy-2-deoxyguanosine: a biomarker of in vivo oxidatve DNA damage［J］． Free Rad Biol Med，1991，10( 3 /4) : 211-216．
［5］ Floyd R A，Watson J J，Wong P K，et al． Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanisms of formation［J］． Free Rad Res Commun，1986，1( 3) : 163-172．
［6］ Guyton K Z，Kensler T W． Oxidative mechanisms in carcinogenesis［J］． Mutat Res，1993，49( 3) : 523-526．
［7］ La D K，Swenberg J A． DNA adducts: biological markers of exposure and potential applications to risk assessment［J］． Mutat Res，1996，365( 1 /3) : 129-146．［8］ Fraga C G，Shigenaga M K，Park J W，et al． Oxidative damage to DNA during aging: 8-hydroxy-2’-deoxyguanosine in rat organ DNA and urine［J］． Proc Natl Acad Sci USA，1990，87( 12) : 4 533-4 537．
［9］ Suzuki H，Inoue Y，Suzuk I S． Changes in the urinary excretion level of 8-hydroxyguanine by exposure to reactive oxygengenerating substances［J］． Free Rad Biol Med，1995，18( 3) : 431-436．
［10］ Loft S，Vistisen K，Ewertz M，et al． Oxidative DNA damage estimated by 8-hydroxy-deoxy-8-hydroxy-deoxyguanosine excretion in humans: influence of smoking，gender and body mass index［J］． Carcinogenesis，1992，13( 12) : 2 241-2 247．
［11］ Li T H，Jia W L，Wang H S，et al． Electrochemical performance of 8-hydroxy-2’-deoxyguanosine and its detection at poly ( 3-methylthiophene) modified glassy carbon electrode［J］． Bisens，Bioelectron，2007，22: 1 245-1 250．
［12］ Wang Y H，Li J，Liu Y，et al． Fabrication of the DNA/poly( 3-methylthiophene) composite film modified electrode and its application for the study on the voltammetric behavior and determination of 8-hydroxy-2’-deoxyguanosine［J］． Sci China Ser B-Chem，2009，52( 11) : 2 006-2 012．
［13］ Iijim a S． Helicalm icrotubules of graphitic carbon［J］． Nature， 1991，354: 56-58．
［14］ 高盐生，董江庆，徐晓燕． 纳米技术在生物传感器中的研究应用［J］． 江苏化工， 2008，36( 3) : 4-6．
［15］ Merz A． Bard A J． A stable surface modified platinum electrode prepared by coating with electroactive polymer［J］． J Am Chem Soc，1978，100( 10) : 3 222-3 223．
［16］ 徐幸梓，王必本，刘天模． 碳纳米管性能的研究进展［J］． 材料导报， 2001， 15( 11) : 44-45．
［17］ Zekra M，Johan B，Andrzej L，et al． Poly( 3，4 -ethylenedioxythiophene) ( PEDOT) doped with carbon nanotubesas ion-toelectron transducer in polymer membrane-based potassium ion selective electrodes［J］． J Electroanal Chem，2009，633( 1) : 246-252．
［18］ 鲁文红，刘爱玲，王先良，等． 利用高效液相色谱-电化学法检测尿液8-羟基脱氧鸟苷［J］． 广东医学，2006，27( 12) : 1 802-1 803．

Memo

Memo:
-
Last Update: 2013-03-11