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The Immune Responses in Chinese Mitten Crab Eriocheir Sinensis and RAW264.7 Cell Line Challenged With Spiroplasma Eriocheiris(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2012年02期
Page:
77-82
Research Field:
生命科学
Publishing date:

Info

Title:
The Immune Responses in Chinese Mitten Crab Eriocheir Sinensis and RAW264.7 Cell Line Challenged With Spiroplasma Eriocheiris
Author(s):
Meng Qingguo1Huang Yanqing2Jin Mingjian3Gu Wei1Wang Wen1
1.Jiangsu Key Laboratory for Biodiversity & Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
Keywords:
Spiroplasma eriocheirisRAW264. 7cell eriocheir sinensis immune response
PACS:
S945
DOI:
-
Abstract:
Spiroplasma eriocheiris is a new kind of pathogen in aquaculture and causes mass mortality of Chinese mitten crab Eriocheir sinensis,but little is known about the immune responses in E. sinensis challenged with this agent. Although immune responses in invertebrates have been widely studied,spiroplasma-induced innate immune responses have not been reported. The mRNA expressions of anti-lipopolysaccharide factor ( ALF) ,prophenoloxidase ( proPO) ,prophenoloxidase- activation factor ( PPAF) ,pacifastin light chain ( PLC) and peroxiredoxin 6 ( Prx6) immune-related genes were examined in order to estimate the effects of S. eriocheiris on the innate immunity of E. sinensis. All of the mRNA expressions of above genes except for Prx6 were significantly up-regulated after S. eriocheiris challenge. But the mRNA expression of Prx6 was significantly down-regulated after the same treatment. S. eriocheiris not only cause tremor disease of E. sinensis,but also can cause suckling mouse cataract just like Spiroplasma mirum. So we chose RAW264. 7 cell line,a murine macrophage-like cell,to study the relationship between the S. eriocheiris and the host in cell level. The expression levels of CD40、IL-1β、IL-10 in RAW264. 7 cells were up-regulated after challenge with S. eriocheiris,while the expression levels of TGF-β1 and TNF-α were down-regulated. The results suggested that broad-spectrum immunere-sponses could be induced by S. eriocheir in both E. sinensis and RAW264. 7cell.

References:

[1] Wang W,Chen J X,Du K H,et al. Morphology of spiroplasmas in the Chinese mitten crab Eriocheir sinensis associated with tremor disease[J]. Research in Microbiology,2004,155( 8) : 630-635.
[2] Wang W,Wen B H,Gasparich G E,et al. A spiroplasma associated with tremor disease in the Chinese mitten crab ( Eriocheir sinensis) [J]. Microbiology,2004,150( 9) : 3 035-3 040.
[3] Wang W,Rong L W,Gu W,et al. Study on experimental infections of Spiroplasma from the Chinese mitten crab in crayfish, mice and embryonated chickens[J]. Research in Microbiology,2003,154( 10) : 677-680.
[4] Wang W,Gu W,Gasparich G E,et al. Spiroplasma eriocheiris sp. nov. ,a novel species associated with mortalities in Eriocheir sinensis,Chinese mitten crab[J]. International Journal of Systematic and Evolutionary Microbiology,2011,61( 3) : 2 745-2 752.
[5] Gasparich G E,Whitcomb R F,Dodge D,et al. The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade[J]. International Journal of Systematic and Evolutionary Microbiology,2004,54( 3) : 893-918.
[6] Regassa L B,Gaspar ich G E. Spiroplasmas: evolutionary relationships and biodiversity[J]. Frontiers in Bioscience,2006, 11( 9) : 2 983-3 002.
[7] Megraud F,Gamon L B,McGarrity G J. Characterization of Spiroplasma mirum ( suckling mouse cataract agent) in a rabbit lens cell culture[J]. Infection and Immunity,1983,42( 3) : 1 168-1 175.
[8] Bastian F O,Sanders D E,Forbes W A,et al. Spiroplasma spp. from transmissible spongiform encephalopathy brains or ticks induce spongiform encephalopathy in ruminants[J]. Journal of Medical Microbiology,2007,56( 9) : 1 235-1 242.
[9] Li C H,Zhao J M,Song L S,et al. Molecular cloning,genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis[J]. Developmental and Comparative Immunology,2008,32( 7) : 784-794.
[10] Gai Y C,Zhao J M,Song L S,et al. A prophenoloxidase from the Chinese mitten crab Eriocheir sinensis: gene cloning, expression and activity analysis[J]. Fish and Shellfish Immunology,2008,24( 2) : 156-167.
[11] 陈大显. 中华绒螯蟹表达序列标签( EST) 分析及免疫相关基因的克隆、表达模式研究[D]. 上海: 华东师范大学生命科学学院,2009.
[12] Gai Y C,Wang L L,Song L S,et al. cDNA cloning,characterization and mRNA expression of a pacifastin light chain gene from the Chinese mitten crab Eriocheir sinensis[J]. Fish and Shellfish Immunology,2008,25( 5) : 657-663.
[13] Mu C K,Zhao J M,Wang L L,et al. Molecular cloning and characterization of peroxiredoxin 6 from Chinese mitten crab Eriocheir sinensis[J]. Fish and Shellfish Immunology,2009,26( 6) : 821-827.
[14] Nunan L M,Lightner D V,Oduori M A,et al. Spiroplasma penaei sp. nov. ,associated with mortalities in Penaeus vannamei, Pacific white shrimp[J]. International Journal of Systematic and Evolutionary Microbiology,2005,55( 6) : 2 317-2 322.
[15] Nunan L M,Pantoja C R,Salazar M,et al. Characterization and molecular methods for detect ion of a novel spiroplasma pathogenic to Penaeus vannamei[J]. Diseases of Aquatic Organisms,2004,62( 3) : 255-264.
[16] Gai Y C,Wang L L,Zhao J M,et al. The construction of a cDNA library enriched for immune genes and the analysis of 7535 ESTs from Chinese mitten crab Eriocheir sinensis[J]. Fish and Shellfish Immunology,2009,27( 6) : 684-694.
[17] Zhao D X,Song S,Wang Q,et al. Discovery of immune-related genes in Chinese mitten crab ( Eriocheir sinensis) by expressed sequence tag analysis of haemocytes[J]. Aquaculture,2009,287( 3 /4) : 297-303.
[18] Tanaka S,Nakamura T,Morita T,et al. Limulus anti-LPS factor: An anticoagulant which inhibits the endotoxin-mediated activation of coagulation system[J]. Biochemical and Biophysical Research Communications,1982,105( 2) : 717-723.
[19] Somboonwiwat K,Marcos M,Tassanakajon A,et al. Recombinant expression and anti-microbial activity of anti-lipopolysaccharide factor ( ALF) from the black tiger shrimp Penaeus monodon[J]. Developmental and Comparative Immunology, 2005,29( 10) : 841-851.
[20] Liu H P,Jiravanichpaisal P,Soderhall I,et al. Antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish Pacifastacus leniusculus[J]. Journal of Virology,2006,80( 21) : 1 0365-1 0371.
[21] Dong C,Zhao J,Song L S,et al. The immune responses in Chinese mitten crab Eriocheir sinensis challenged with doublestranded RNA[J]. Fish and Shellfish Immunology,2009,26( 3) : 438-442.
[22] Simonet G,Claeys I,Broeck J V. Structural and functional properties of a novel serine protease inhibiting peptide family in arthropods[J]. Comparative Biochemistry and Physiology,2002,132( 1) : 247-255.
[23] Lu K Y,Huang Y T,Lee H H,et al. Cloning the prophenoloxidase cDNA and monitoring the expression of proPO mRNA in prawns ( Macrobrachium rosenbergii) stimulated in vivo by CpG oligodeoxynucleotides[J]. Fish and Shellfish Immunology, 2006,20( 3) : 274-284.
[24] Ko C F,Chiou T T,Vaseeharan B,et al. Cloning and characterisation of a prophenoloxidase from the haemocytes of mud crab Scylla serrata[J]. Developmental and Comparative Immunology,2007,31( 1) : 12-22.
[25] Chen P,Li J,Li J,et al. Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunustri tuberculatus [J]. Fish and Shellfish Immunology,2010,28( 1) : 106-112.
[26] Wood Z A,Schrder E,Robin H J,et al. Structure,mechanism and regulation of peroxiredoxins[J]. Trends in Biochemical Sciences,2003,28( 1) : 32-40.[27] Zhang Q,Li F,Zhang J,et al. Molecular cloning,expression of a peroxiredoxin gene in Chinese shrimp Fenneropenaeus chinensis and the antioxidant activity of its recombinant protein[J]. Molecular Immunology,2007,44( 14) : 3 501-3 509.
[28] Maningas B,Koyama T,Kondo H,et al. A peroxiredoxin from kuruma shrimp,Marsupenaeus japonicus,inhibited by peptidoglycan [J]. Developmental and Comparative Immunology,2007,32( 3) : 198-203.
[29] Rawadi G,Roman-Roman S. Mycoplasma membrane lipoproteins induced proinflammatory cytokines by a mechanism distinct from that of lipopolysaccharide[J]. Infection and Immunity,1996,64( 2) : 637-643.
[30] Razin S,Yogev D,Naot Y. Molecular biology and pathogenicity of mycoplasmas[J]. Microbiology and Molecular Biology Reviews,1998,62: 1 094-1 156.[31] Rottem S. Interaction of mycoplasmas with host cells[J]. Physiological Reviews,2003,83( 2) : 417-432.

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Last Update: 2013-03-11