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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:

2012年02期

Page:

89-92

Research Field:

生命科学

Publishing date:

Info

Title:

Comparison of DNA Extraction Methods From Leaves of Momordica Charantia(Cucurbitaceae)

Author(s):

Ji Yuan¹; ²; Hou Beiwei¹; Luo Yuming¹; ²

1.School of Life Sciences,Nanjing Normal University,Nanjing 210046,China

Keywords:

Momordica charantia Linn． ; DNA extraction; modified mehods of CTAB; SRAP

PACS:

S642.5

DOI:

-

Abstract:

Because of polysaccharides and polyphenolic and other interfering compounds in leaves of Momordica charantia ( Cucurbitaceae) ，the genomic DNA extraction of methods namly modified CTAB，Dneasy Kit and CTAB were compared． Then the quality and purified of extracted DNA was detected，and high quality DNA was measured by sequencerelated amplified polymorphism ( SRAP) markers． The results showed that modified CTAB moethod could be used to extract DNA from leaves of M． charantia，with high qulity，high purify，high relative molecular mass and low interfering compounds． SPAP markers detection was also shown clear bands and good polymorphism． So，the modified CTAB method was suitable for the extraction of genomic DNA from M． charantia，which was also available to further SRAP or other molecular markers study．

References:

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［10］ Singh A K，Behera T K，Chandel D，et al． Assessing genetic relationships among bitter gourd ( Momordica charantia L． ) accessions using inter-simple sequence repeat ( ISSR) markers［J］． J Hortic Sci Biotech，2007，82( 2) : 217-222．
［11］ 温庆放，李大忠，朱海生，等． 不同来源苦瓜遗传亲缘关系RAPD 分析［J］． 福建农业学报，2005，20( 3) : 185-188．
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