|Table of Contents|

Comparison of Ultrastructure of Callus Induction in Indica Rice With Different Callus Induction Rates(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年01期
Page:
68-73
Research Field:
生命科学
Publishing date:

Info

Title:
Comparison of Ultrastructure of Callus Induction in Indica Rice With Different Callus Induction Rates
Author(s):
Tian Qinqin1; 2; Yan Lina2; Lu Changmei1; Li Xia2; Wei Xiaodong2; Fang Xianwen2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.Institute of Foods Crop,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R&D Center,Nanjing 210014,China)
Keywords:
Indica rice; mature embryo; callus tissue; ultrastructure
PACS:
S511
DOI:
-
Abstract:
Mature embryos from 3 Indica rice varieties as explants were implanted in the induction medium(M8+30 g/L Sucrose+10 g/L Agar+5 g/L Sorbitol+2 mg/L 2,4-D+2 mg/L 6-BA+1 mg/L ABA+0.5 mg/L KT+1 mg/L NAA)and the callus inductivities of these materials were different significantly at 0.01 level,respectively.At the same time,the callus incubated for 9 d and 15 d were observed by a transmission electron microscope,and the callus’browning rate was recorded at the same time.The results showed that the 3 rice materials developed differently at the same induction period.The callus cells from Yangfuxian 6 developed fast,while those from Hui 76 developed relatively slow,and the development of callus cells from Huanghuazhan was the slowest.The performances in callus ultrastructure were also consistent with their cell development periods.It suggested that the callus induction in Indica rice had close relation with their cell development rates,physiological functions and senescences as well.The callus browning was also related to the accumulation period of some black substance and the development of their vacuoles.The rate of callus induction in Indica rice would be increased by adding cytokinin in medium for callus induction.

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Last Update: 2013-03-31