|Table of Contents|

Preparation and Identification of Polyclonal Antibodies of GGPPS(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年01期
Page:
94-98
Research Field:
生命科学
Publishing date:

Info

Title:
Preparation and Identification of Polyclonal Antibodies of GGPPS
Author(s):
Wang Jiangnan1; 4; Ding Yao1; 4; Lai Shanshan1; 4; Chen Weibo2; Yu Decai2; Li Chaojun2; 3; Xue Bin2; 3
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.School of Medicine,Nanjing University,Nanjing 210093,China) (3.Jiangsu Key Laboratory for Molecular Medicine,Nanjing 210093,China) (4.Jiangsu Key Laboratory for Molecular and Me
Keywords:
GGPPS; prokaryotic expression; fusion protein; polyclonal antibody
PACS:
Q7
DOI:
-
Abstract:
To construct the prokaryotic expression vector of human GGPPS gene and prepare the specific polyclonal antibody against GGPPS.Construct the prokaryotic expression vector of pGEX-4T-GGPPS by DNA recombination technology and induce the expression of GGPPS fusion protein.The fusion protein was purified and injected into rabbits to produce polyclonal antibody.The prokaryotic expression vector of pGEX-4T-GGPPS was constructed and the antibody of GGPPS was obtained.The titer of the antiserum was 1:32 000.The specific polyclonal antibody against GGPPS was successfully prepared.The polyclonal antibodies preparation against GGPPS provided a good tool for us to study the function involvement of GGPPS.

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Last Update: 2013-03-31