|Table of Contents|

Improvement of the Chromosome-based Recombineering System(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年01期
Page:
99-103
Research Field:
生命科学
Publishing date:

Info

Title:
Improvement of the Chromosome-based Recombineering System
Author(s):
Fan Dan; Shi Mudan; Yang Yunwen; Shang Guangdong
Jiangsu Engineering and Technology Research Center for Microbiology,Jiangsu Key Laboratory for Microbes and Functional Genomics,School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
recombineering; knock-out; recombination efficiency; SSOR; DSBR
PACS:
Q819
DOI:
-
Abstract:
Recombineering is a genetic engineering technology for the DNA cloning and modification based on the recombinase-catalyzed homologous recombination.In this study,starting from previous obtained LS-GR,a chromosome-based recombineering strain,the 5’->3’ single stranded DNA exonuclease coding gene recJ and exonucleolytic cleavage in the 3’->5’ DNA exonuclease coding gene sbcB were knocked out via LS-GR’s recombineering function as well as its inherent double strand break repair(DSBR)function.Compared with LS-GR,the obtained strains LS2002 and LS2004 show higher single strand oligonucleotide repair(SSOR)activity and DSBR activities,showing that they have the potential to be used in the recombineering experiments for high recombination efficiency.

References:

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Last Update: 2013-03-31