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Generation of PP2A Cα Knockout Model in Neonatal Mouse Ventricular Myocytes(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年02期
Page:
74-77
Research Field:
生命科学
Publishing date:

Info

Title:
Generation of PP2A Cα Knockout Model in Neonatal Mouse Ventricular Myocytes
Author(s):
Chen Wen; Wen Mingda; Dong Dachuan; Gao Yanhong; Zhang Zhao
Jiangsu Key Laboratory for Molecular and Medical Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
PP2A Cα; neonatal mouse ventricular myocytes; Cre-adenovirus
PACS:
Q2-33
DOI:
-
Abstract:
To generate PP2A Cα knockout model in neonatal mouse ventricular myocytes.Male PP2A Cαfl/fl,Cre/- were selectively mated with female PP2A Cαfl/fl,Cre/- mice to obtain abundant offsprings with the same genotype.The neonatal mouse ventricular myocytes were digested by trypsinization and subsequently infected with Cre-adenovirus.The effects of adenovirus infection were observed by fluorescence microscope and identified by western blot analysis.The offsprings in aboundance were obtained by seting up a mating between male and female of PP2A Cαfl/fl,Cre/-mice,as a result,an abundance of neonatal mouse ventricular myocytes with the genotype of PP2A Cαfl/fl,Cre/-were available for Ad-Cre infection.Green fluorescence was observed after GFP labeled Cre-adenovirus(Cre-Ad)infection for 48 h.The expression level of PP2A Cα protein in Cre-Ad infected myocytes was 60%～80% lower than control.The PP2A Cα knockout model in neonatal mouse ventricular myocytes was successfully generated.

References:

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Memo

Memo:
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Last Update: 2013-06-30