|Table of Contents|

Immune Responses of Eriocheir Sinensis and RAW264.7 Cells Induced by Spiralin-like Protein SLP25 from Spiroplasma Eriocheiris(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年02期
Page:
78-83
Research Field:
生命科学
Publishing date:

Info

Title:
Immune Responses of Eriocheir Sinensis and RAW264.7 Cells Induced by Spiralin-like Protein SLP25 from Spiroplasma Eriocheiris
Author(s):
Meng Qingguo1; Chen Jing1; Huang Yanqing2; Jin Mingjian3; Gu Wei1; Wang Wen1
1.Jiangsu Key Laboratory for Biodiversity and Biotechnology and Jiangsu Key Laboratory for Aquatic Crustacean Diseases, School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
2.Key and Open Laboratory of Marine and Estuary Fisheries,Ministry of Agriculture of China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences,Shanghai 200090,China
3.Rudong Station for Fisheries Technology Extension,Rudong 226400,China
Keywords:
Spiroplasma; Chinese mitten crab; Spiralin; immune responses
PACS:
Q936
DOI:
-
Abstract:
Spiroplasma eriocheiris caused mass mortality of Chinese mitten crab Eriocheir sinensis.This paper studied the immune responses of E.sinensis and RAW264.7 cells induced by a unique gene,spiralin-like protein of S.eriocheiris.After cloning and expression the spiralin-like protein in E.coli,the role of this protein in promoting immune responses was characterized through analyzing the interactions between S.eriocheiris and E.sinensis as well as RAW264.7 cells.Real-time PCR and western blot were used for detection of expression levels of immune genes in both hosts.The mRNA expressions of anti-lipopolysaccharide factor,prophenoloxidase,pacifastin light chain and peroxiredoxin 6 in E.sinensis and TNF-α,IL-1β,IL-10 in RAW264.7 cells were induced by spiralin-like protein,whereas TGF-β1 and CD40 in RAW264.7 cells were not.In RAW264.7 cells,the levels of phosphorylated P38 and phosphorylated ERK increased after spiralin-like protein induced,whereas the level of phosphorylated JNK did not change.This study reveals that spiralin-like protein of S.eriocheiris can induce the immune responses of E.sinensis and RAW264.7 cells and the protein may be an important virulence factor of S.eriocheiris.This study gives light to the pathogenesis of the spiroplasmas and provides knowledge of immune reactions of hosts from both invertebrate and vertebrate challenged with spiroplasmas unique protein.

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Last Update: 2013-06-30