|Table of Contents|

Microsatellite Analysis on the Genetic Structure of Three Breeding Stocks of Eriocheir Sinensis(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年02期
Page:
84-90
Research Field:
生命科学
Publishing date:

Info

Title:
Microsatellite Analysis on the Genetic Structure of Three Breeding Stocks of Eriocheir Sinensis
Author(s):
Tang Liuxiu1; 2; Xu Zhiqiang2; Ge Jiachun1; 2; 3; Chen Yirong4; Zhao Muzi2; Yang Jiaxin1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
2.Freshwater Fisheries Research Institute of Jiangsu Province,Nanjing 210017,China
3.Nanjing Purity Aquaculture Development Co.,Ltd.,Gaochun 211316,China
4.Gaochun Gucheng Shijiu Lakes Administration Committee,Gaochun 211300,China
Keywords:
Eriocheir sinensis; microsatellite; base stocks for breeding; genetic diversity
PACS:
Q953
DOI:
-
Abstract:
In order to select excellent germplasm of Eriocheir sinensis,16 pairs of microsatellite primers were used to analyze the genetic characters of 3 base stocks which were collected from Chongming Tuanjiesha of Yangtze River Delta,Yangzhong section,and Jingjiang,Liuhe,Jiang Pu sections of Yangtze River respectively.The results showed that the genetic diversity indices of the 3 stocks are high with average expected heterozygosities(He)as 0.701 4,and the average observed heterozygosities(Ho)of the 3 stocks were higher than the average expected heterozygosities(He)in the all 16 microsatellite loci.The average observed heterozygosities(Ho)of the 3 stocks were 0.775 0,0.745 7 and 0.791 7,and the average expected heterozygosities(He)were 0.705 0,0.702 9,and 0.696 4 respectively.The first stock had the highest He,while the third stock had the lowest value,but there was no significant difference among the three stocks(P>0.05).The fixation indices(Fis)were used to evaluate the inbreeding or outcrossing of the 3 stocks,and the negative values were observed at almost all 16 microsatellite loci for the 3 stocks.The Hardy-Weinberg equilibrium(D)among the three stocks ranged from -0.379 4 to 0.966 6,and there were heterozygous reduction at five locus in each of these stocks.The pairwised genetic distance among the three stocks ranged from 0.032 1 to 0.038 4.The Ds between the first and second stock was smaller(0.032 1)than any others and it can be inferred that the genetic exchange between them was more frequent.Overall,the three base stocks in the present study had relatively higher genetic diversities,and it was suitable for selective breeding.

References:

[1] 赵乃刚.长江中华绒螯蟹种质资源混杂对养蟹业的影响[J].内陆水产,1998,5:2-4.
[2]王成辉,李思发.中华绒螯蟹种质研究进展[J].中国水产科学,2002,9(1):92-96.
[3]王武.我国中华绒螯蟹养殖的现状和发展前景[J].内陆水产,1998,4:2-4.
[4]Dunham R A,Brummett R E.Response of two generations of selection to increased body weight in channel catfish,Ictalurus punctatus,compared to hybridization with blue catfish,I.furcatus,males[J].J Appl Aquac,1999,9:37-45.
[5]Gjerde B.Growth and reproduction in fish and shellfish[J].Aquaculture,1986,57:37-55.
[6]Myers J M,Heggelund P O,Hudson G,et al.Genetics and broodstock management of coho salmon[J].Aquaculture,2001,197:43-62.
[7]Fleming I A,Gross M R.Breeding success of hatchery and wild coho salmon(Oncorhynchus kisutch)in competition[J].Ecol Appl,1993,3(2):230-245.
[8]Eknath A E,Tayamen M M,Palada-de Vera M S,et al.Genetic improvement of farmed tilapias:the growth erformance of eight strains of Oreochromis niloticus tested in rent farm environments[J].Aquaculture,1993,111:171-188.
[9]Longalong F M,Eknath A A,Bentsen H B.Response to bidirectional selection for frequency of early maturing females in Nile tilapia(Oreochromis niloticus)[J].Aquaculture,1999,178:13-25.
[10]Ponzoni R W,Hamzah A,Tan S,et al.Genetic parameters and response to selection for five weight in the GIFT strain of Nile tilapia(Oreochromis niloticus)[J].Aquaculture,2005,247:203-210.
[11]颉晓勇,李思发,蔡完其.吉富品系尼罗罗非鱼选育过程中遗传变异的微卫星分析[J].水产学报,2007,31(3):385-390.
[12]桂建芳.鱼类性别和生殖的遗传基础及其人工控制[M].北京:科学出版社,2007:166-167.
[13]鲁翠云,曹顶臣,孙效文,等.微卫星分子标记辅助镜鲤家系构建[J].中国水产科学,2008,15(6):893-901.
[14]Hänfling B,Weetman D.Characterization of microsatellite loci for the Chinese mitten crab,Eriocheir sinensis[J].Mol Ecol Notes,2003,3:15-17.
[15]Ma H T,Chang Y M,Yu D M,et al.Microsatellite variations among four populations of Eriocheir sinensis[J].Zoological Research,2007,28(2):126-133.
[16]徐成,王可玲,张培军.鲈鱼群体生化遗传学研究Ⅱ.种群生化遗传结构及变异[J].海洋与湖沼,2001,32(3):248-254.
[17]Frankham R,Ballou J,Briscoe D.Introduction to Conservation Genetics[M].Cambridge:Cam bridge University Press,2010,260-308.
[18]Chang Y M,Liang L Q,Ma H T,et al.Microsatellite analysis of genetic diversity and population structure of Chinese Mitten Crab(Eriocheir sinensis)[J].Journal of Genetics and Genomics,2008,35:171-176.
[19]潘建林,牟大凯,郝莎,等.中华绒螯蟹Eriocheir sinensis两个地理种群的微卫星DNA多态性分析[J].南京大学学报:自然科学版,2006,42(5):457-461.
[20]许志强,葛家春,李跃华,等.四水系中华绒螯蟹天然群体遗传特征的微卫星标记分析[J].南京大学学报:自然科学版,2011,47(1):82-90.
[21]葛家春,许志强,李晓晖,等.利用线粒体COI序列分析4水系中华绒螯蟹群体遗传学特征[J].中国水产科学,2011,18(1):16-22.
[22]李晓晖,许志强,潘建林,等.中华绒螯蟹人工选育群体的遗传多样性[J].中国水产科学,2010,17(2):236-242.
[23]许加武,任明荣,李思发.长江、辽河、瓯江中华绒螯蟹种群的形态判别[J].水产学报,1997,21(3):269-274.
[24]王武,张文博.绒螯蟹三个种群形态判别比较[J].水产科技情报,2005,32(2):81-83.
[25]郑曙明,吴青.中华绒螯蟹同工酶的研究[J].水生生物学报,1994,18(2):183-185.
[26]周开亚,高志千.RAPD标记鉴别中华绒螯蟹种群初步研究[J].应用与环境生物学报,1999,5(2):176-180.
[27]Zouros E.On the relation between heterozygosity and heterosis:an evaluation of the evidence from marine mollusks[J].Isozymes Current Topics in Biological and Medical Research,1987,15(3):255-270.

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Last Update: 2013-06-30