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Changes of the Ubiquitinated Proteins Stressed Under the Deltamethrin in Diamondback Moth,Plutella Xylostella L.(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年02期
Page:
108-112
Research Field:
生命科学
Publishing date:

Info

Title:
Changes of the Ubiquitinated Proteins Stressed Under the Deltamethrin in Diamondback Moth,Plutella Xylostella L.
Author(s):
Du Yaqiong1; Zhang Hong1; Li Fengliang2; Cheng Luogen1
1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
2.Institute of Plant Protection,Guizhou Academy of Agriculture Science,Guiyang 550006,China
Keywords:
Plutella xylostella; Ubiquitin gene family; RNAi; gene silencing
PACS:
S481+4
DOI:
-
Abstract:
The aim of this study was to analyse the Ubiquitinated protein in the 4rd larvae of Plutella xylostella under the stress of deltamethrin using SDS-PAGE,Western Blot and LC MS/MS.The results revealed significant differences in Ubiquitinated protein expression between deltamethrin-resistant strain and deltamethrin-sensitive strain of Plutella xylostella.It is successfully proved the differences in Ubiquitinated protein expression of Plutella xylostella under the stress of deltamethrin.The study provides a reference for analysing the function of Ubiquitin in the insect cells and the regulation function of it in physiology,biochemistry and environmental stress.

References:

[1] Ciechanover A.The ubiquitin proteolytic system and pathogenesis of human diseases:a novel platform for mechanism-based drug targeting[J].Biochem Soc Trans,2003,31(2):474-481.
[2]Hoeller D,Dikic I.Targeting the ubiquitin system in cancer therapy[J].Nature,2009,458(7237):438-444.
[3]Pickart C M,Eddins M J.Ubiquitin:structures,functions,mechanisms[J].Biochim Biophys Acta(BBA)-Mol Cell Res,2004,1695(1):55-72.
[4]Shelton A,Wyman J,Cushing N,et al.Insecticide resistance of diamondback moth(Lepidoptera:Plutellidae)in North America[J].J Econ Entomol,1993,86(1):11-19.
[5]谢苗,成娟,尤民生,等.基于质谱和生物信息学分析的小菜蛾蛋白质鉴定[J].昆虫学报,2009,52(11):1 206-1 212.
[6]Liu C,Li F,Han Z.Studies on deltamethrin resistance breeding and its resistant mechanism of diamond back moth[J].Acta Phytophy Sin,1995,22(4):367-372.
[7]Weill M,Berthomieu A,Berticat C,et al.Insecticide resistance:a silent base prediction[J].Curr Biol:CB,2004,14(14):R552.
[8]Bates S L,Zhao J Z,Roush R T,et al.Insect resistance management in GM crops:past,present and future[J].Nat Biotechnol,2005,23(1):57-62.
[9]张娜娜,许勤,李风良,等.小菜蛾成虫蛋白质组双向电泳图谱的建立及条件优化[J].南京师大学报:自然科学版,2011,34(2):78-82.
[10]Yun E Y,Goo T W,Hwang J S,et al.Bombyx mori ubiquitin mRNA[J].GenBank Accession No AF308163,2000.
[11]于航,金丰良,许小霞,等.德国小蠊泛素基因的克隆及序列分析[J].昆虫学报,2004,47(4):522-525.
[12]Cheng L G,Xu J N,Xue J,et al.Downregulation of ubiquitin gene expression in the deltamethrin-resistant diamondback moth(Plutella xylostella L.)[J].Appl Entomol,2009,133(7):533-538.
[13]黄强,尹沛源,路鑫,等.色谱-质谱联用技术在代谢组学中的应用[J].色谱,2009,27(5):566-572.
[14]Huo J,Shi H,Yao Q,et al.Cloning and purification of recombinant silkworm dihydrolipoamide dehydrogenase expressed in Escherichia coli[J].Protein Expression Purif,2010,72(1):95-100.
[15]刘凤沂,须志平,薄仙萍,等.昆虫抗药性与适合度[J].昆虫知识,2008,45(3):374-378.

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Last Update: 2013-06-30