|Table of Contents|

Establishment of High Frequency Regeneration System of Phytolacca Americana(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
74-80
Research Field:
生命科学
Publishing date:

Info

Title:
Establishment of High Frequency Regeneration System of Phytolacca Americana
Author(s):
Wu Haojie1; Yang Jie1; Xu Yali1; Yang Ziyi1; Zheng Tiesong2; Lu Changmei1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.Ginling College,Nanjing Normal University,Nanjing 210097,China)
Keywords:
Phytolacca americana Linn.; regeneration system; explants; adventitious buds; rooting; medium
PACS:
Q945.5
DOI:
-
Abstract:
Phytolacca americana Linn.,which belongs to Phytolaccaceae,is a kind of perennial herb with high application value in industry,agriculture and medicine.In order to establish a stable and efficient regeneration system for P.americana,efficiency of callus induction,adventitious bud differentiation and elongation,and adventitious root induction etc.were analyzed from aspects of explants selection,hormones combination and applications of GA3 and AgNO3 etc..Results show that,the optimal explants for callus induction and bud differentiation are stem sections; the method of transplanting shoots in induction medium to sound seedling can significantly advance adventitious bud differentiation and promote bud proliferation coefficient.The optimal medium for adventitious bud differentiation is MS+6-BA 3.0 mg/L+NAA 0.01 mg/L+TDZ 0.02 mg/L with a multiplication coefficient up to 12.7.The optimal medium for adventitious buds elongation is MS+TDZ 0.02 mg/L+6-BA 3.0 mg/L+NAA 0.01 mg/L+GA3 2.0 mg/L.The optimal rooting medium is 1/2MS+NAA 0.4 mg/L with a rooting rate up to 91.7%.AgNO3 treatment inhibited the adventitious bud differentiation of P.americana.

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Last Update: 2013-09-30