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Inhibition Effects of the Proliferation and Induction of the Apoptosis Of Celastrol In C2C12 Cells(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
93-96
Research Field:
生命科学
Publishing date:

Info

Title:
Inhibition Effects of the Proliferation and Induction of the Apoptosis Of Celastrol In C2C12 Cells
Author(s):
Xu Yanhua; Liu Jin; Chen Huaqun
Jiangsu Key Laboratory for Molecular and Medical Biotechnology,School of Life Sciences, Nanjing Normal University,Nanjing 210023,China
Keywords:
Celastrol; C2C12 myoblast; cell proliferation; cell apoptosis
PACS:
Q28
DOI:
-
Abstract:
Celastrol is an effective content extracted from god vine,a traditional herb.It has been widely used in the treatment of arthritics and nephritis.Recently,it has further been found to suppress the proliferation of tumor cells,and as a potential candidate to treat tumor.We report in this study that in vitro,Celastrol inhibits the proliferation of mouse myoblast,C2C12 cells,as well as induces apoptosis through activation of Caspase-3.Our datas suggest that the safety of Celastrol is needed to be further investigated.

References:

[1] Li H,Zhang Y Y,Tan H W,et al.Therapeutic effect of tripterine on adjuvant arthritis in rats[J].J Ethnopharmacol,2008,118(3):479-484.
[2]陈铭祥,冯玉静,王定勇,等.雷公藤红素的研究进展[J].中成药,2010,32(3):473-476.
[3]雷万军,王建军.雷公藤免疫抑制作用研究概况[J].洛阳医专学报,1996,15(2):129-133.
[4]Yu X,Tao W,Jiang F,et al.Celastrol attenuates hypertension-induced inflammation and oxidative stress in vascular smooth muscle cells via induction of heme oxygenase-1[J].Am J Hypertens,2010,23(8):895-903.
[5]Yang H,Chen D,Cui Q C,et al.Celastrol,a triterpene extracted from the Chinese "Thunder of God Vine" is a potent proteasome inhibitor and suppresses human prostate cancer growth in nude mice[J].Cancer Res,2006,66(9):4 758-4 765.
[6]鲍一笑,张登海,张玲珍,等.雷公藤红素诱导CEM-6T细胞凋亡的机制研究[J].上海免疫学杂志,2003,23(3):187-189.
[7]Mou H,Zheng Y,Zhao P,et al.Celastrol induces apoptosis in non-small-cell lung cancer A549 cells through activation of mitochondria- and Fas/FasL-mediated pathways[J].Toxicol in Vitro,2011,25(5):1 027-1 032.
[8]Vermes I,Haanen C,Steffens-Nakken H,et al.A novel assay for apoptosis:Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V[J].J Immunol Methods,1995,184(1):39-51.
[9]David Y,Ora S.Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle[J].Nature,1977,270(5639):725-727.
[10]Philippe G,Wei H Y,Raymond A S.Ischemic preconditioning by caspase cleavage of poly(ADP-Ribose)polymerase-1[J].The Journal of Neuroscience,2003,23(22):7 967-7 973.
[11]徐银海,严杰.雷公藤红素诱导人急性髓系白血病HL-60细胞凋亡及其机制的研究[J].浙江大学学报:理学版,2008,35(3):311-314.
[12]Lu W,Jia G,Meng X,et al.Beta-catenin mediates the apoptosis induction effect of celastrol in HT29 cells[J].Life Sci,2012,91(7/8):279-283.
[13]Zhou L L,Lin Z X,Fung K P,et al.Celastrol-induced apoptosis in human HaCaT keratinocytes involves the inhibition of NF-κB activity[J].Eur J Pharmacol,2011,670(2/3):399-408.
[14]Kannaiyan R,Manu K A,Chen L,et al.Celastrol inhibits tumor cell proliferation and promotes apoptosis through the activation of c-Jun N-terminal kinase and suppression of PI3 K/Akt signaling pathways[J].Apoptosis,2011,16(10):1 028-1 041.
[15]罗伟,陈卫昌.雷公藤红素联合5-氟尿嘧啶在人结肠癌细胞中的相互作用[J].中国现代医药杂志,2008,10(12):4-7.

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Last Update: 2013-09-30