|Table of Contents|

Cloning and Tissue Expression Analyses of MRF4 Gene in Trachidermus Fasciatus(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
108-115
Research Field:
生命科学
Publishing date:

Info

Title:
Cloning and Tissue Expression Analyses of MRF4 Gene in Trachidermus Fasciatus
Author(s):
Zhang Yanping; Shao Fang; Zheng Hongmei; Lu Xiangyun; Xu Jianrong; Gu Zhiliang
College of Biological Science and Food Technology,Changshu Institute of Technology,Changshu 215500,China
Keywords:
Trachidermus fasciatus; MRF4; RACE; tissue expressional pattern
PACS:
Q38; S917.4
DOI:
-
Abstract:
The transcription factor MRF4 is one member of MyoD family which have essential functions in myogenic lineage determination and muscle differentiation.In order to obtain full-length MRF4 gene sequence and characterize its tissue expression profile in Trachidermus fasciatus,RT-PCR and rapid-amplification cDNA ends(RACE)were used.A full-length of 1049 bp MRF4 cDNA,which contained a 720 bp long complete ORF encoding a 239 amino acid peptide.Multiple sequence comparison showed that the T.fasciatus MRF4 had a high homology with Epinephelus coioides at 91.81%,followed by Takifugu rubripes.A homology with mammals and birds was at about 63%,and of the lowest homology with Xenopus laevis at 59.65%.The bHLH domain of T.fasciatus MRF4 was forming α-helix dimer that like a scissors,and HLH sequence was highly homologous among different species.MRF4 gene was detected in all 6 examined tissues,but its expression level in muscle was significantly higher than the amount of other tissues,implying that the fish MRF4 gene would play an important role in muscle development.

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Last Update: 2013-09-30