|Table of Contents|

Cloning and Analysis of Ubiqitin Genes of Plutella Xylostella(L.)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2013年03期
Page:
116-119
Research Field:
生命科学
Publishing date:

Info

Title:
Cloning and Analysis of Ubiqitin Genes of Plutella Xylostella(L.)
Author(s):
Zhang Hong1; Du Yaqiong1; Li Fengliang2; Zhou Zhou1; Gu Shuyu1; Cheng Luogen1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.Institute of Plant Protection,Guizhou Academy of Agriculture Science,Guiyang 550006,China)
Keywords:
Plutella xylostella; ubiquitin gene family; RNAi; gene silencing
PACS:
S481+4
DOI:
-
Abstract:
The cDNA sequences of ubiquitin genes were isolated from Plutella xylostella by reverse transcription polymerase chain reaction(RT-PCR).Using RNA interference(RNAi),the in vitro systhesized double-stranded RNA(dsRNA)homologous to the UBA52 gene was microinjected into 4th instar larvae of P.xylostella to surpress the gene expression.The results of semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR)demonstrated that UBA52 gene expression was reduced sharply at 72h after injection.The ubiquitin gene was silenced successfully in this experiment,and the establishment of RNAi technique can help reveal the gene function of P.xylostella and other insects.

References:

[1] Goldstein G,Scheid M,Hammerling U,et al.Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells[J].Proc Natl Acad Sci USA,1975,72(1):11-15.
[2]Cabrera H,Barrio R,Arribas C.Structure and expression of the Drosophila ubiquitin-52-amino-acid fusion-protein gene[J].Biochem J,1992,286(1):281-288.
[3]Barrio R,Arco A D,Cabreia H L,et al.Structure and expression of the Drosophila ubiquitin-80-amino-acid fusion-protein gene[J].Biochem J,1994,302(1):237-244.
[4]Pfeifer K,Frank W,Schroder H C,et al.Cloning of the polyubiquitin cDNA from the marine sponge Geodia cydonium and its preferential expression during reaggregation of cells[J].J Cell Sci,1993,106:545-553.
[5]Von Kampen J,Nielander U,Wettern M.Expression of ubiquitin genes in Chlamydomonas renhardtii:involvement in stress response and cell cycle[J].Planta,1995,197:528-534.
[6]Li Z F,Pang Y,Yu J X.Cloning and sequencing of ubiquitin gene from Spodoptera litura[J].Entomologia Sinica,2003,10(1):27-34.
[7]于航,金丰良,许小霞,等.德国小蠊泛素基因的克隆及序列分析[J].昆虫学报,2004,47(4):522-525.
[8]李朝飞,于航,潘丽晶,等.棉铃虫泛素基因的克隆及序列分析[J].中山大学学报,2005,44(1):61-64.
[9]Cheng L G,Xu J N,Xue J,et al.Downregulation of ubiquitin gene expression in the deltamethrin-resistant diamondback moth(Plutella xylostella L.)[J].J Appl Entomol,2009,133:533-538.
[10]Pickart C M.Back to the future with ubiquitin[J].Cell,2004,116(2):181-190.
[11]Ober K A,Jockusch E L.The roles of wingless and decapentaplegic in axis and appendage development in the red flour beetle,Tribolium castaneum[J].Dev Biol,2006,294(2):391-405.
[12]Zhou X G,Wheeler M M,Oi F M,et al.RNA interference in the termite reticulitermes flavipes through ingestion of double-stranded RNA[J].Insect Biochem Molec,2008,38:805-815.
[13]Zhuang S F,Lisha K,James B N.Multiple alpha subunits of integrin are involved in cell-mediated responses of the Manduca immune system[J].Dev Comp Immunol,2008,32(4):365-379.

Memo

Memo:
-
Last Update: 2013-09-30