|Table of Contents|

The Season and Weather Impacts on Noninvasive Genetic Sampling of the David’s Deer(Elaphurus davidianus)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2014年01期
Page:
123-
Research Field:
生命科学
Publishing date:

Info

Title:
The Season and Weather Impacts on Noninvasive Genetic Sampling of the David’s Deer(Elaphurus davidianus)
Author(s):
Wang Yan1; Qian Chen1; Ding Jingjing1; 2; Chen Ya1; Liu Jiaxiuyu1;  Ding Yuhua3; Zhu Lifeng1; Chang Qing1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.Jiangsu Academy of Forestry,Nanjing 211153,China) (3.Jiangsu Dafeng Milu National Nature Reserve,Dafeng 224136,China)
Keywords:
noninvasive genetic sampling; Elaphurus davidianus; OD 260/280; DNA quality
PACS:
Q953
DOI:
-
Abstract:
In recent years,noninvasive genetic sampling plays an important role in genetic researches of many endangered animals.When we used this method to analyze the genetic diversity of David’s Deer,Elaphurus davidianus,one of the world endangered species,we found the DNA quality from fecal samples which collected in winter was better than that in summer.The proportion of OD 260/280 ratio which between 1.60 and 1.90 was about 61.57% for the winter samples,and about 43.51% for the summer samples,according to the standard procedure of the QIAamp DNA Stool Mini Kit.The success rate of PCR amplification for gender identification and microsatellite genotyping was 84.98% and 96.25% for winter samples,and 93.13% and 95.25% for summer samples respectively.There was no significant difference between the different seasons.The weather such as rain and snow influenced the DNA quality significantly.The proportion of OD 260/280 ratio which between 1.60 and 1.90 was about 61.57% for the sunny winter samples,about 23.68% and 23.88% for the samples after raining and snowing respectively.The rain would wash out the intestinal tissue,mucosa or blood on the surface of fecal samples,and made the degradation of the DNA.The results suggest that the noninvasive genetic sampling for David’s Deer should be done in sunny winter,as it can improve the DNA quality effectively.

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Last Update: 2014-03-30