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Optimization of Ultrasonic-Assisted Extracting and Free Radicals Scavenging of Polysaccharides from Blood Pummelo Peel(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2014年01期
Page:
141-
Research Field:
生命科学
Publishing date:

Info

Title:
Optimization of Ultrasonic-Assisted Extracting and Free Radicals Scavenging of Polysaccharides from Blood Pummelo Peel
Author(s):
Chen Wenjuan1; Chen Jianfu2
(1.Department of Food Engineering,Zhangzhou City University,Zhangzhou 363000,China) (2.College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou 350108,China)
Keywords:
blood pummelo peel; polysaccharides; ultrasonic-assisted; free radicals scavenging
PACS:
R284.2; O636.1; TS201
DOI:
-
Abstract:
The polysaccharides from blood pummelo peel was abstracted by ultrasonic-assisted heat extraction,and the extraction yield was measured by coloration method with phenol-sulfuric acid.The effects of extraction yield on the parameters including ultrasonic power,ultrasonic temperature,material-to-liquid ratio,ultrasonic time were investigated by single factor test and orthogonal array design methods.And the notability difference was analyzed with the statistic method of range and variance.Results showed that the optimal conditions were as follows:ultrasonic power was 80 W,ultrasonic temperature was 65 ℃,material-to-liquid ratio was 1:30,ultrasonic time was 70 min.The extraction yield of the polysaccharides from blood pummelo peel was reached up to 18.51% and it increased 2.24% compared to the heat extraction without ultrasonic-assisted.Hydroxyl radical scavenging ability,DPPH scavenging ability and reducing capacity of polysaccharide were studied by taking ascorbic acid as a referential substance.The result showed that it had good free radicals scavenging activity,however,weaker antioxidant activity than ascorbic acid.These results clearly established the possibility that polysaccharide from blood pummelo peel should be effectively employed as the type of natural antioxidant.

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Last Update: 2014-03-30