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Induction of the Cytotoxicity and the Relative Genes Expression Response of Deltamethrin in Drosophila Kc cells(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2014年02期
Page:
106-
Research Field:
生命科学
Publishing date:

Info

Title:
Induction of the Cytotoxicity and the Relative Genes Expression Response of Deltamethrin in Drosophila Kc cells
Author(s):
Jiao Dongxu; Li Na; Cheng Chen; Ge Chunnan; Cheng Luogen
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
deltamethrin; cytotoxicity; real鄄time quantitative PCR; western blotting
PACS:
S481+4
DOI:
-
Abstract:
Deltamethrin(DM)is a kind of nerve agent,and commonly used in controlling crop pests,because of the high efficacy,low toxicity. Widespread and improper use of DM induced insect resistance,but the resistance mechanism is not clear at present. Cytotoxicity analysis reveals that the cells proliferation, cellular morphology and cells viability have significant changes under DM stress. Too Low or too high concentration of DM could obviously inhibit the activity of cells. Under 15 ppm treatment, cell activity was higher. Real-time quantitative PCR and western-blotting analysis demonstrated that the genes associated with oxidative stress and immune responses were induced sharply under DM stress. Our research focuses on the cytotoxicity and gene differential expression,these results can provide basis for the study of molecular toxicology and mechanism of deltamethrin resistance.

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Last Update: 2014-06-30