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cDNA Library Construction and Analysis of Differentially Expressedin Roots of Robinia pseudoacacia Induced by Rhizobium 87-1-1(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2014年04期
Page:
76-
Research Field:
生命科学
Publishing date:

Info

Title:
cDNA Library Construction and Analysis of Differentially Expressedin Roots of Robinia pseudoacacia Induced by Rhizobium 87-1-1
Author(s):
Zhao Yinjuan; Sun Dunping; Han Sufen; Wu Xiaoqin
Southern China Collaborative Innovation Center of Sustainable Forestry,College of Forestry,Nanjing Forestry University,Jiangsu Key Laboratory for Prevention and Management of Invasion Species,Nanjing 210037,China
Keywords:
Rhizobium; Robinia pseudoacacia; transfer cells; SSH; function analysis
PACS:
S718
DOI:
-
Abstract:
To find special genes in transfer cells of Locust which was differentiated from root epidemic cells induced by rhizobium,a forward and reverse suppression subtraction hybridization(SSH)cDNA library were constructed successfully.Using cDNA from the roots of Robinia pseudoacacia induced by rhizobium as the tester and cDNA from roots of Locust untreated as driver seperately,suppression subtraction hybridization libraries was constructed.500 colonies in both libraries were isolated and sequenced.Functions of ESTs were analyzed by blast in NCBI including database Nt,Nr,Swissprot,COG,Interpro and GO after removing repeat and redundancy sequences.725 uniESTs including 385 uniESTs in forward library and 340 uniESTs in reverse library were obtained.The assembling provided a total of 51 contigs and 674 singletons by cluster analyses of the uniESTs.Nucleotide homology searched with Blastn in NCBI non-redundant nucleotide database and 674 uniESTs(93% of total uniESTs)were homologous with known genes.Protein homology searched with Blastx in NCBI non-redundant protein database and 648 uinESTs(89% of total uniESTs)were homologous with known proteins.The results of gene ontology(GO)annotation showed that 369 uinESTs were involved in biological process with 270 times,molecular function with 448 times and cell component with 484 times respectively.Among these ESTs,putative proteins were related to posttranslational modification,transcription factors,cell signals,cell wall/cell membrane/endomembrane system,cytoskeleton and nodulin genes in positive library,and related to growth and metabolites biosynthesis in reverse library.These results were generally consistent with physiological processes when the Robinia pseudoacacia were induced by rhizobium.Such as MYB transcription factor,Vesicle-associated membrane protein could be found in positive library which might be special expression in the transfer cells to aid the understanding of development of transfer cells.

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Last Update: 2014-12-31