|Table of Contents|

Effects of Estrogen on Sweet-Associated Protein Expression in the Intestine of Mice(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2015年02期
Page:
98-
Research Field:
生命科学
Publishing date:

Info

Title:
Effects of Estrogen on Sweet-Associated Protein Expression in the Intestine of Mice
Author(s):
Li Guoliang1; Chen Mengling2; Zheng Min2; Wang Bing2; Geng Qianqian2; Zhang Yuxin3;  Liang Pei2; Zhan Yuehua2; Zhang Genhua1; 2
(1.School of Biology and Basic Medical Sciences,Soochow University,Suzhou 215123,China) (2.School of Biological and Food Engineering,Changshu Institute of Technology,Changshu 215500,China) (3.Changshu High School of Jiangsu Province,Changshu 215500,China)
Keywords:
ovariectomy; estrogen; intestines; C57BL/6 mice
PACS:
Q28
DOI:
-
Abstract:
Estrogen has been known to be associated with the perception of sweetness,which can modify people’s intake of carbohydrates by affecting the perception of sweetness. However,the mechanism of how is the estrogen involved in the intestinal taste processing has not been fully understood. In this study,we have established the mouse model of estrogen deficiency,by the means of ovariectomy. The consequent modifications of the sweet taste transduction related signal expression have been analyzed on molecular and protein levels in intestine with qRT-PCR and Western blot technique. The results show that,with ovaries removed mice,estrogen levels in blood decreased markedly and the body weight increased significantly. Meanwhile,the expression of the duodenum T1R3 has been reduced,and those of T1R2 and TRPM5 have been inhibited to some extend as well. This study suggests that the estrogen plays an important role in mouse intestinal taste perception.

References:

[1] McLaughlin S K,McKinnon P J,Margolskee R F. Gustducin is a taste-cell-specific G protein closely related to the transducins[J]. Nature,1992,357(6 379):563-569.
[2]Busque S M,Kerstetter J E,Geibel J P,et al. L-type amino acids stimulate gastric acid secretion by activation of the calcium-sensing receptor in parietal cells[J]. Am J Physiol Gastrointest Liver Physiol,2005,289(4):G664-G669.
[3]Hira T,Nakajima S,Eto Y,et al. Calcium-sensing receptor mediates phenylalanine-induced cholecystokinin secretion in enteroendocrine STC-1 cells[J]. Febs J,2008,275(18):4 620-4 626.
[4]Dyer J,Salmon K S,Zibrik L,et al. Expression of sweet taste receptors of the T1R family in the intestinal tract and enteroendocrine cells[J]. Biochem Soc Trans,2005,33(Pt 1):302-305.
[5]Bezencon C,le Coutre J,Damak S. Taste-signaling proteins are coexpressed in solitary intestinal epithelial cells[J]. Chem Senses,2007,32(1):41-49.
[6]Hofer D,Puschel B,Drenckhahn D. Taste receptor-like cells in the rat gut identified by expression of alpha-gustducin[J]. Proc Natl Acad Sci USA,1996,93(13):6 631-6 634.
[7]Hass N,Schwarzenbacher K,Breer H. A cluster of gustducin-expressing cells in the mouse stomach associated with two distinct populations of enteroendocrine cells[J]. Histochem Cell Biol,2007,128(5):457-471.
[8]Bezencon C,Furholz A,Raymond F,et al. Murine intestinal cells expressing Trpm5 are mostly brush cells and express markers of neuronal and inflammatory cells[J]. J Comp Neurol,2008,509(5):514-525.
[9]Carr D B,Utzschneider K M,Hull R L,et al. Intra-abdominal fat is a major determinant of the National Cholesterol Education Program Adult Treatment Panel Ⅲ criteria for the metabolic syndrome[J]. Diabetes,2004,53(8):2 087-2 094.
[10]Ferrara C M,Lynch N A,Nicklas B J,et al. Differences in adipose tissue metabolism between postmenopausal and perimenopausal women[J]. J Clin Endocrinol Metab,2002,87(9):4 166-4 170.
[11]Tchernof A,Despres J P. Sex steroid hormones,sex hormone-binding globulin,and obesity in men and women[J]. Horm Metab Res,2000,32(11/12):526-536.
[12]dos Reis C M,de Melo N R,Meirelles E S,et al. Body composition,visceral fat distribution and fat oxidation in postmenopausal women using oral or transdermal oestrogen[J]. Maturitas,2003,46(1):59-68.
[13]Curtis K S,Stratford J M,Contreras R J. Estrogen increases the taste threshold for sucrose in rats[J]. Physiol Behav,2005,86(3):281-286.
[14]Frye C A,Crystal S,Ward K D,et al. Menstrual cycle and dietary restraint influence taste preferences in young women[J]. Physiol Behav,1994,55(3):561-567.
[15]Carey D G,Jenkins A B,Campbell L V,et al. Abdominal fat and insulin resistance in normal and overweight women:direct measurements reveal a strong relationship in subjects at both low and high risk of NIDDM[J]. Diabetes,1996,45(5):633-638.
[16]Naaz A,Zakroczymski M,Heine P,et al. Effect of ovariectomy on adipose tissue of mice in the absence of estrogen receptor alpha(ERalpha):a potential role for estrogen receptor beta(ERbeta)[J]. Horm Metab Res,2002,34(11/12):758-763.
[17]Stubbins R E,Holcomb V B,Hong J,et al. Estrogen modulates abdominal adiposity and protects female mice from obesity and impaired glucose tolerance[J]. Eur J Nutr,2012,51(7):861-870.
[18]Nelson G,Hoon M A,Chandrashekar J,et al. Mammalian sweet taste receptors[J]. Cell,2001,106(3):381-390.
[19]Li X,Staszewski L,Xu H,et al. Human receptors for sweet and umami taste[J]. Proc Natl Acad Sci USA,2002,99(7):4 692-4 696.
[20]Adler E,Hoon M A,Mueller K L,et al. A novel family of mammalian taste receptors[J]. Cell,2000,100(6):693-702.
[21]Mace O J,Affleck J,Patel N,et al. Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2[J]. J Physiol,2007,582(Pt 1):379-392.
[22]Mace O J,Lister N,Morgan E,et al. An energy supply network of nutrient absorption coordinated by calcium and T1R taste receptors in rat small intestine[J]. J Physiol,2009,587(Pt 1):195-210.
[23]Le Gall M,Tobin V,Stolarczyk E,et al. Sugar sensing by enterocytes combines polarity,membrane bound detectors and sugar metabolism[J]. J Cell Physiol,2007,213(3):834-843.
[24]Jang H J,Kokrashvili Z,Theodorakis M J,et al. Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1[J]. Proc Natl Acad Sci USA,2007,104(38):15 069-15 074.
[25]Margolskee R F,Dyer J,Kokrashvili Z,et al. T1R3 and gustducin in gut sense sugars to regulate expression of Na+-glucose cotransporter 1[J]. Proc Natl Acad Sci USA,2007,104(38):15 075-15 080.
[26]Wong G T,Gannon K S,Margolskee R F. Transduction of bitter and sweet taste by gustducin[J]. Nature,1996,381(6 585):796-800.
[27]Dotson C D,Roper S D,Spector A C. PLCbeta2-independent behavioral avoidance of prototypical bitter-tasting ligands[J]. Chem Senses,2005,30(7):593-600.
[28]Damak S,Rong M,Yasumatsu K,et al. Trpm5 null mice respond to bitter,sweet,and umami compounds[J]. Chem Senses,2006,31(3):253-264.
[29]Glendinning J I,Bloom L D,Onishi M,et al. Contribution of alpha-gustducin to taste-guided licking responses of mice[J]. Chem Senses,2005,30(4):299-316.
[30]Brixel L R,Monteilh-Zoller M K,Ingenbrandt C S,et al. TRPM5 regulates glucose-stimulated insulin secretion[J]. Pflugers Arch,2010,460(1):69-76.

Memo

Memo:
-
Last Update: 2015-06-30