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InfluenceofNRAGEGeneonMouseHippocampusNerveCellDifferentiation(PDF)

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2015年03期
Page:
56-
Research Field:
生命科学
Publishing date:

Info

Title:
InfluenceofNRAGEGeneonMouseHippocampusNerveCellDifferentiation
Author(s):
ZhouXiaotianWangMinLiuZhenzhouLiHuiQiuHaiwenLiuMei
SchoolofLifeSciences,NanjingNormalUniversity,JiangsuProvinceKeyLaboratoryforMolecularandMedicalBiotechnology,Nanjing210023,China
Keywords:
NRAGEprimaryneurondifferentiationlow-densitycultures
PACS:
Q254
DOI:
-
Abstract:
NRAGE,whichbelongstoMAGEfamily,playsanimportantroleinthecellcycle,proliferationandapoptosis.TheinfluenceofNRAGEondifferentiationofneuronshadbeenreportedintwopaperswhichindicatedtwooppositeconclusions.ThesetwoarticlesbasedonPC12cells,whichderivedfromapheochromocytomaoftheratadrenalmedulla.OurexperimentisolatedhippocampalsofNRAGEknockoutmiceandtookneonatalratglialcellsasfeederlayertoobtainfetalmiceprimaryneurons.DetectedtheinfluenceofNRAGEgeneonneuronsdifferentiationbycountingneurite/diameterofsomaandthenumbersofdendrites.Theresultsshowedthatprimaryneuronaldifferentiationsofwild-typeandknockoutfetalmicehadnosignificantdifference,indicatingNRAGEgenehasnoeffectontheneuronsofmice.

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Last Update: 2015-09-30