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Study on Anti-Tumor Activity and Mechanisms of Blf-ADT(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2016年01期
Page:
67-
Research Field:
生命科学
Publishing date:

Info

Title:
Study on Anti-Tumor Activity and Mechanisms of Blf-ADT
Author(s):
Wang Qian; Pan Wang; Li Wei; Xiao Zhiyi; Pan Lili; Xu Guanglin
School of Life Sciences，Nanjing Normal University，Jiangsu Province Key Laboratory for Molecularand Medical Biotechnology，Nanjing 210023，China
Keywords:
HeLa; Blf-ADT; cell proliferation; cell apoptosis; anti-tumor effect
PACS:
Q4
DOI:
-
Abstract:
The purpose of this study was to observe the activity and mechanisms of a new compound Blf-ADT on HeLa cells. The effects of Blf-ADT on Hela cells was investigated by morphological observation，MTT assay and colony formation assay，respectively. Wound healing assay was used to detect the effect of Blf-ADT on the migration of HeLa cells. Meanwhile，the activities of Blf-ADT on apoptosis was measured by Flow cytometry. Furthermore，Western blot was performed to investigate the expressions of related proteins in HeLa cell line treated with Blf-ADT. As a result，we found that Blf-ADT could inhibit cell proliferation and reduce cell migration in a dose-and time-dependent manner. In addition，Blf-ADT could significantly increase the rate of apoptosis and affect the expression of apoptosis-related proteins. Taken together，the data indicate that Blf-ADT can significantly inhibit the growth of HeLa cells and have potential clinical value for treatment of cervical cancer.

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Memo

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Last Update: 2016-03-30