|Table of Contents|

Molecular Mechanism of Phloretin-Induced Apoptosisin Prostate Cancer LNCaP Cells(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2017年02期
Page:
43-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Molecular Mechanism of Phloretin-Induced Apoptosisin Prostate Cancer LNCaP Cells
Author(s):
Kang Dan; Wu Zhaomeng; Cao Runyi; Wang Fangfang; Tian Qian; Liu Ping
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
phloretin; cell apoptosis; cell cycle; PI3K/AKT signal pathway; prostate cancer
PACS:
Q291
DOI:
10.3969/j.issn.1001-4616.2017.02.008
Abstract:
Phloretin,a natural product found in pears,apples and various vegetables,exerts anti-inflammatory,anti-oxidant and anti-tumor effects. Here,we investigated the molecular mechanism of phloretin-induced apoptosis in prostate cancer LNCaP cells. In this study,effects of phloretin on the viability of prostate cancer cells were investigated by morphological observation,MTT assay and CCK-8 assay,respectively. Effect of phloretin on apoptosis and cell cycle was measured by Flow Cytometry. Furthermore,western blot was employed to investigate the levels of phosphorylation of PI3K and AKT(the activity of PI3K/AKT signaling pathway); and check the expression of cell apoptosis-related and cell cycle-related proteins which were downstream of PI3K/AKT signaling pathway in LNCaP cells. As a result,we found that phloretin significantly blocked cell cycle arrest in G2/M phase by decreasing the protein level of Cyclin B1,and decreased the cell viability and increased the nuclear rupture in a dose-dependent manner; and finally resulted in a significantly apoptosis in LNCaP cells. In molecular level,phloretin showed a dose-dependent down-regulation of the activity of PI3K/AKT via down-regulating the phosphorylation of PI3K and AKT proteins,and then regulated the expression of cell apoptosis-related and cell cycle-related proteins. Therefore,phloretin significantly inhibited cell viability and promoted apoptosis in prostate cancer cells,suggesting that phloretin had potential clinical value for treatment of prostate cancer in clinic.

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Last Update: 2017-06-30