|Table of Contents|

Characterization and Comparative Analysis of MicroRNAs During EmbryonicDevelopment Between the Chinese Mitten Crab(Eriocheir sinensis)and the Freshwater Crab(Sinopotamon yangtsekiense)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2017年02期
Page:
65-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Characterization and Comparative Analysis of MicroRNAs During EmbryonicDevelopment Between the Chinese Mitten Crab(Eriocheir sinensis)and the Freshwater Crab(Sinopotamon yangtsekiense)
Author(s):
Sun Bin; Liu Yingying; Li Peng; Yan Jie; Zhou Kaiya
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnologyand Jiangsu Key Laboratory for Aquatic Crustacean Diseases,Nanjing 210023,China
Keywords:
Eriocheir sinensis; Sinopotamon yangtsekiense; microRNA; miRNA
PACS:
Q951+4
DOI:
10.3969/j.issn.1001-4616.2017.02.011
Abstract:
The deep sequencing of small RNA in Eriocheir sinensis and Sinopotamon yangtsekiense embryo samples was performed using the Illumina/Solexa high-throughput sequencing technologies. After analyzing the biological information of the sequencing results,8 569 743 and 8 719 465 clean unique sequence data were screened in E.sinensis and S.yangtsekiense respectively,and the length distribution in the range of 20 nt-22 nt was 30.47%(Ejs-OB:E.sinensis embryonic sequencing library)and 30.38% respectively(SY-OJC:S.yangtsekiense embryonic sequencing library). Mapping the small RNA tags to genome by SOAP to analyze their distribution on the genome,5 745 279 and 4 397 509 small RNAs of Ejs-OB and SY-OJC were screened respectively,13 472(Ejs-OB)and 18 407(SY-OJC)of which were genome-matched reads. Annotated small RNA tags with different kinds of RNA,the results showed that a total of 6 293 445 and 5 596 614 miRNA candidate reads were searched in Ejs-OB and SY-OJC respectively. However,the novel miRNAs were not found. Expression profile analysis found that the expression level of miR-1183,miR-1357,miR-1591,miR-2382 were up-regulated and the difference were significant. Interestingly,some same miRNAs* sequences were also searched in the two library,and miR-139*,miR-1419g*,miR-1798*,miR-454* and miR-2068* of which were also have a significant differences in expression. These results indicated that these miRNAs may be involved in regulating gene expression associated with the development of the embryo in E.sinensis and S.yangtsekiense.

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Last Update: 2017-06-30