|Table of Contents|

The Construction of Mevalonate Pathway Enzyme GGPPSProtein Binding Molecules Screening Model(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2017年04期
Page:
87-
Research Field:
·生命科学·
Publishing date:

Info

Title:
The Construction of Mevalonate Pathway Enzyme GGPPSProtein Binding Molecules Screening Model
Author(s):
Qi Yaling1; Lu Wuguang2; Chong Danyang1; Liu Jia1; Sun Qian1; Xu Xiaojun3; Cao Peng2; Fang Lei1; Li Chaojun1; Xue Bin1
(1.State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Key Laboratory of Molecular Medicine,School of Medicine,Nanjing University,Nanjing 210093,China)(2.Jiangsu Province Hospital on Integration of Chinese and Western Medicine,Nanjing 21002
Keywords:
GGPPS recombination protein; microscale thermophoresis; small molecular screening
PACS:
Q291
DOI:
10.3969/j.issn.1001-4616.2017.04.014
Abstract:
The post-translational modifications of proteins include phosphorylation,glycosylation,acetylation,palmitic acid and so on. And the prenylation modification of proteins is one of the basic post-translational lipid modifications of proteins. The key enzyme of mevalonate pathway,GGPPS,can catalyze FPP to generate GGPP and mediate the equilibrium of protein prenylation,affect disease progression including insulin resistance,hyperyension,abnormal reproductive metabolism. Hence,it has an important practical significance that construct a GGPPS interaction model to obtain its molecule therapeutic drugs. Obtain the high expression of EGFP fluorescent labeled GGPPS prokaryotic purified protein. Construct a GGPPS interaction model with microscale thermophoresis technology. The recombinant expression plasmid GGPPS-EGFP-His was constructed correctly verified by gel electrophoresis and sequencing verification.The purified recombinant protein reached a purity of about 80%. In the microscale thermophoresis assay,the GGPPS substrate farnesyl pyrophosphate concentration gradient test showed a good combination.

References:

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Last Update: 2017-12-30