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Identification and Analysis of Differentially Expressed Proteins inAngiotensin Ⅱ Induced-Mouse Model of Cardiac Hypertrophy(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年03期
Page:
78-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Identification and Analysis of Differentially Expressed Proteins inAngiotensin Ⅱ Induced-Mouse Model of Cardiac Hypertrophy
Author(s):
Feng Meng; Zhu Xinran; Zheng Yingying; Zhang Zhao; Zhang Yin
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
cardiac hypertrophy; proteomics; mitochondria; energy metabolism
PACS:
R331.3+1
DOI:
10.3969/j.issn.1001-4616.2018.03.013
Abstract:
To identify the differential expression of proteins in Angiotensin Ⅱ(Ang Ⅱ)induced-mouse model of cardiac hypertrophy and explore the pathophysiological significance. A mouse model of cardiac hypertrophy was established by subcutaneous administration of Ang Ⅱ with an osmotic pump-based controlled release system and then the cardiac proteins were extracted. The differentially expressed proteins were separated with two dimensional electrophoresis(2-DE)and analyzed by matrix assisted laser desorption ionization/time-of-flight mass spectrometry(MALDI-TOF-MS)and the original data were processed by Mascot then searched in NCBInr database. There were significant differences in the expression levels of 34 proteins between Ang Ⅱ and Control groups(P<0.05),14 differentially expressed proteins were identified according to the database,in which 12 were down-regulated and 2 were up-regulated. The differentially expressed proteins in Ang Ⅱ induced-mouse model of cardiac hypertrophy were mainly related to energy metabolism,oxidative stress and cytoskeleton.

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Last Update: 2018-11-19