|Table of Contents|

Resveratrol Ameliorate Oxidative Stress afterExperimental Subarachnoid Hemorrhage(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2018年03期
Page:
95-
Research Field:
·生命科学·
Publishing date:

Info

Title:
Resveratrol Ameliorate Oxidative Stress afterExperimental Subarachnoid Hemorrhage
Author(s):
Yang Li; Yu Qing; Zhang Chunbing
Jiangsu Provincial Hospital of Traditional Chinese Medicine,Nanjing University of Chinese Medicine,Nanjing 210029,China
Keywords:
subarachnoid hemorrhage; resveratrol; oxidative stress; SIRT1; PGC-1α
PACS:
R310
DOI:
10.3969/j.issn.1001-4616.2018.03.015
Abstract:
It has been reported that resveratrol(RSV)can ameliorate injury after subarachnoid hemorrhage(SAH). The aim here is to elucidate the antioxidant function of RSV after SAH. In experimental 1,brain SIRT1 and PGC-1α protein levels were examined in different groups after SAH induction. In experimental 2,rats were divided into 5 groups:sham group,SAH group,SAH+vehicle group,SAH+RSV group,and SAH+RSV+EX527 group. The SIRT1 activator RSV and specific inhibitor EX527 were respectively administered. Then,Western blot,Immunofluorescence staining,oxidant/antioxidant factors,brain edema,and neurologic scoring were performed. After experimental SAH was induced,the nuclear SIRT1 and PGC-1α protein level increased from 6 h to 12 h,with a peak at 24 h,but gradually decreased from 48 h. RSV treatment in SAH rats led to a significant increment of SIRT1 and PGC-1α protein levels resulting in an enhancement of antioxidant effect when compared with SAH rats without treatment. The antioxidant effect of RSV in SAH rats could be partially abrogated by the SIRT1 inhibitor EX527. These results show that RSV has an antioxidant effect after SAH via the SIRT1/PGC-1α signaling pathway.

References:

[1] MACDONALD R L,SCHWEIZER T A. Spontaneous subarachnoid haemorrhage[J]. Lancet,2017,389(10069):655-666.
[2]MCCUBREY J A,LERTPIRIYAPONG K,STEELMAN L S,et al. Regulation of GSK-3 activity by curcumin,berberine and resveratrol:potential effects on multiple diseases[J]. Advances in biological regulation,2017,65:77-88.
[3]MARKUS M A,MORRIS B J. Resveratrol in prevention and treatment of common clinical conditions of aging[J]. Clin Intervaging,2008,3(2):331-339.
[4]KORONOWSKI K B,KHOURY N,SAUL I,et al. Neuronal SIRT1(Silent Information Regulator 2 Homologue 1)regulates glycolysis and mediates resveratrol-induced ischemic tolerance[J]. Stroke,2017,48(11):3117-3125.
[5]HAM P R,RAJU R. Mitochondrial function in hypoxic ischemic injury and influence of aging[J]. Prog Neurobiol,2017,157:92-116.
[6]WANG Z,MA C,MENG C,et al. Melatonin activates the Nrf2-ARE pathway when it protects against early brain injury in a subarachnoid hemorrhage model[J]. J Pineal Res,2012,53(2):129-137.
[7]周晓明. SIRT1/P53通路在蛛网膜下腔出血后脑水肿机制中作用的初步探讨[D]. 上海:第二军医大学,2014.
[8]ZHOU X M,ZHOU M L,ZHANG X S,et al. Resveratrol prevents neuronal apoptosis in an early brain injury model[J]. J Surg Res,2014,189(1):159-165.
[9]YAN W,FANG Z,YANG Q,et al. SirT1 mediates hyperbaric oxygen preconditioning-induced ischemic tolerance in rat brain[J]. J Cereb Blood Flow Metab,2013,33(3):396-406.
[10]张翔圣,范杰梅,张鑫,等. 虾青素对兔蛛网膜下腔出血后的保护作用研究[J]. 中华神经外科疾病研究,2015(1):18-21.
[11]肖继东,李晓峰,韦俊杰,等. 褪黑素对脑出血大鼠的脑保护作用及机制[J]. 临床神经病学,2014(6):450-454.
[12]YAMAGUCHI M,ZHOU C,NANDA A,et al. Ras protein contributes to cerebral vasospasm in a canine double-hemorrhage model[J]. Stroke,2004,35(7):1750-1755.
[13]SEHBA F A,HOU J,PLUTA R M,et al. The importance of early brain injury after subarachnoid hemorrhage[J]. Prog Neurobiol,2012,97(1):14-37.
[14]PIGNATARO G,ESPOSITO E,SIRABELLA R,et al. nNOS and p-ERK involvement in the neuroprotection exerted by remote postconditioning in rats subjected to transient middle cerebral artery occlusion[J]. Neurobiol Dis,2013,54:105-114.
[15]ZAKHARY S M,AYUBCHA D,DILEO J N,et al. Distribution analysis of deacetylase SIRT1 in rodent and human nervous systems[J]. The anatomical record:advances in integrative anatomy and evolutionary biology,2010,293(6):1024-1032.
[16]ZHANG X,WU Q,WU L,et al. Sirtuin 1 activation protects against early brain injury after experimental subarachnoid hemorrhage in rats[J]. Cell Death Dis,2016,7(10):e2416.
[17]LIU C,LIN J D. PGC-1 coactivators in the control of energy metabolism[J]. ACTA Bioch Bioph Sin,2011,43(4):248-257.
[18]ZHANG F,WU Y. Resveratrol may be an effective prophylactic agent for ischemic stroke[J]. J formos Med Assoc,2011,110(8):485-486.
[19]MARX W,KELLY J T,MARSHALL S,et al. Effect of resveratrol supplementation on cognitive performance and mood in adults:a systematic literature review and meta-analysis of randomized controlled trials[J]. Nutr Rev,2018,76(6):432-443.

Memo

Memo:
-
Last Update: 2018-11-19