|Table of Contents|

Colorimetric Method for Efficient Determination ofPoly-γ-glutamic Acid in Fermentation Broth(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2019年02期
Page:
105-108
Research Field:
·生命科学·
Publishing date:

Info

Title:
Colorimetric Method for Efficient Determination ofPoly-γ-glutamic Acid in Fermentation Broth
Author(s):
Liu Pengli; Liu Ping; Huang Chen; Yin Zhimin
School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
Keywords:
poly-γ-glutamic acid; methylene blue; colorimetric method; quantitative analysis
PACS:
Q5-33
DOI:
10.3969/j.issn.1001-4616.2019.02.017
Abstract:
A colorimetric method for the rapid determination of γ-PGA in fermentation broth was established. The principle is that poly-γ-glutamic acid can react with the methylene blue solution and change the color of the methylene blue solution. Different concentrations of γ-PGA standard solutions were prepared to form a reaction system with methylene blue solution,and the effects of methylene blue solution concentration,reaction temperature,and reaction time on the reaction system were compared and optimized. The results showed that the mass concentration of methylene blue solution was 10 mg/L,the reaction temperature was 30 ℃,the reaction time was 5 min,the linear regression equation was y=0.002 4x+0.328 9,R2=0.996 5,and the blank average recovery rate was 106.8%. The average RSD value was 1.51%,the average recoveries in the fermentation broth were 118.3%,and the average RSD was 1.89%. The determination of γ-PGA in fermentation broth by colorimetry is simple and rapid,with good reproducibility,high sensitivity and good accuracy. It can be used to detect γ-PGA concentration in fermentation broth.

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Last Update: 2019-06-30