|Table of Contents|

Study on the Function of Atp10 in Schizosaccharomyces pombe(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2019年02期
Page:
109-114
Research Field:
·生命科学·
Publishing date:

Info

Title:
Study on the Function of Atp10 in Schizosaccharomyces pombe
Author(s):
Xie Wanqiu; Huang Ying
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
Atp10; Schizosaccharomyces pombe; respiration chain; mitochondrial
PACS:
180.612
DOI:
10.3969/j.issn.1001-4616.2019.02.018
Abstract:
Mitochondria are power plants in eukaryotic cells. Most of the energy required for cell life activities comes from oxidative phosphorylation of mitochondria. First,we constructed the strain of atp10 gene deletion by homologous recombination and observed its growth phenotype in non-fermentation medium with glycerol and galactose as the sole carbon source. Then,bioinformatics analyses revealed that Atp10 contains a 32-amino-acids mitochondrial localization sequence(MTS)at the N-terminus. The green fluorescence confirmed that Atp10 is localized in mitochondria. Western blotting assay showed that the deletion of Atp10 resulted in the decrease of the expression of mitochondrial-related proteins and affected the assembly of mitochondrial respiratory chain complex. In short,Atp10 is a protein closely related to mitochondrial function,which is essential for the normal functioning of mitochondrial respiratory chain.

References:

[1] VO T D,PALSSON B O. Building the power house:recent advances in mitochondrial studies through proteomics and systems biology[J]. American journal of physiology-cell physiology,2007,292(1):164-177.
[2]LURIN C1,ANDRéS C,AUBOURG S,et al. Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins reveals their essential role in organelle biogenesis[J]. Plant cell,2004,16(8):2089-2103.
[3]PAPA S,SCACCO S,SCHLIEBS M,et al. Mitochondrial diseases and aging[J]. Molecular aspects of medicine,1996,17(6):513-563.
[4]RAULE N,SEVINI F,SANTORO A,et al. Association studies on human mitochondrial DNA:methodological aspects and results in the most common age-related diseases[J]. Mitochondrion,2007,7(1/2):29-38.
[5]DOWLING D K. Evolutionary perspectives on the links between mitochondrial genotype and disease phenotype.[J]. Biochimica Et Biophysica Acta,2014,1840(4):1393-1403.
[6]SCHON E A,DIMAURO S,HIRANO M. Human mitochondrial DNA:roles of inherited andsomatic mutations[J]. Nature reviews genetics,2012,13(12):878-890.
[7]NEWSHOLME P,GAUDEL C,KRAUSE M. Mitochondria and diabetes:an intriguing pathogenetic role[J]. Advances in experimental medicine and biology,2012(942):235-247.
[8]JAMES A M,COLLINS Y,LOGAN A,et al. Mitochondrial oxidative stress and the metabolic syndrome[J]. Trends in endocrinology and metabolism,2012,23(9):429-434.
[9]HATAKEYAMA H,GOTO Y I. Respiratory chain complex disorganization impairs mitochondrial and cellular integrity:phenotypic variation in cytochrome c oxidase deficiency[J]. American journal of pathology,2017,187(1):110-121.
[10]MAYR J A,HAACK T B,FREISINGER P,et al. Spectrum of combined respiratory chain de fects[J]. Journal of inherited metabolic disease,2015,38(4):629-640.
[11]ROLOFF G A,HENRY M F. Mam33 promotes cytochrome c oxidase subunit I translation in Saccharomyces cerevisiae mitochondria[J]. Molecular biology of the cell,2015,26(16):2885-2894.
[12]CROSS R L. The mechanism and regulation of ATP synthesis by F1-ATPases[J]. Annu Rev Biochem,1981,50:681-714.
[13]WALKER J E,FEARNLEY I M,GAY N J,et al. Primary structure and subunit stoichiometry of F1-ATPase from bovine mitochondria[J]. Journal of molecular biology,1985,184(4):677-701.
[14]FUTAI M,KANAZAWA H. Structure and function of proton-translocating adenosine triphosphatase(F0F1):biochemical and molecular biological approaches[J]. Microbiological reviews,1983,47(3):285-312.
[15]TZAGOLOFF A,MEAGHER P. Assembly of the mitochondrial membrane system. V. properties of a dispersed preparation of the rutamycin-sensitive adenosine triphosphatase of yeast mitochondria[J]. The journal of biological chemistry,1971,246(23):7328-7336.
[16]ALEXANDER T,ANTONI B,WALTER N,et al. Atp10p assists assembly of Atp6p into the Fo unit of the yeast mitochondrial ATPase[J]. The journal of biological chemistry,2004,279(19):19775-19780.
[17]CLAROS M G,VINCENS P. Computational method to predict mitochondrially imported proteins and their targeting sequences[J]. European journal of biochemistry,1996,241(3):779-786.
[18]岳强,周惠. 粟酒裂殖酵母——一种良好的真核模式生物[J]. 韶关学院学报,2003,24(3):100-102.
[19]黄敏. 基因敲除技术及其应用[J]. 广东轻工职业技术学院学报,2009,8(4):12-14.
[20]PAUL M F,BARRIENTOS A,TZAGOLOFF A. A single amino acid change in subunit 6 of theyeast mitochondrial ATPase suppresses a nullmutation in ATP10[J]. The journal of biological chemistry,2000,275(38):29238-29243.
[21]DE J P,VAN DIJKEN J P,PRONK J T. Metabolic fluxes in chemostat cultures of Schizosaccharomyces pombe grown on mixtures of glucose and ethanol[J]. Microbiology,1996,142(6):1399-1407.

Memo

Memo:
-
Last Update: 2019-06-30