|Table of Contents|

Mitochondrial ROS Contributes to Lycorine-inducedApoptosis in HepG2 Cells(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2019年04期
Page:
91-96
Research Field:
·生命科学·
Publishing date:

Info

Title:
Mitochondrial ROS Contributes to Lycorine-inducedApoptosis in HepG2 Cells
Author(s):
Kang Jingjing; Liu Xiaoning
Department of Biochemistry,School of Medicine,Huanghe Science and Technology College,Zhengzhou 450063,China
Keywords:
Lycorine; human hepatoma HepG2 cells; apoptosis; mito-Grx1-roGFP2; mitochondrial ROS; SS31
PACS:
R392.5
DOI:
10.3969/j.issn.1001-4616.2019.04.013
Abstract:
To further explore the underlying apoptotic molecular mechanism of lycorine on HepG2 cells. The growth of HepG2 cells were measured with TransDetect Cell Counting Kit(CCK-8)assay. Apoptosis percentages of cells exposed to lycorine with or without SS31 was determined by flow cytometry. Apoptotic nuclear morphology was assessed using Hoechst 33342. Western blot analysis was used to examine the expression of fragmented PARP. It was successfully established that an human hepatoma cell line HepG2 with mito-Grx1-roGFP2 probe stably expressed which could be used as a real-time reporter of dynamic redox changes. As an antioxidant which could remove the ROS of mitochondria,the treatment of SS31 improved the morphology of HepG2 cells injured by lycorine and inhibited lycorine-induced cell apoptosis. It was also found that SS31 significantly inhibited the fragment of PARP induced by lycorine. Furthermore,SS31 attenuated lycorine-induced rise of 405/488 fluorescence ratio which could reflect the level of ROS in mitochondria. The findings suggest that mitochondrial ROS contribute to lycorine-induced apoptosis in HepG2 cells and lycorine is expected to be a potential drug for the treatment of liver cancer,it worth profund study.

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Last Update: 2019-12-31