|Table of Contents|

Tandem Expression,Purification and Bioactivity Characterization ofBovine Lactoferricin Peptide in Escherichia coli(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2020年02期
Page:
100-107
Research Field:
·生物学·
Publishing date:

Info

Title:
Tandem Expression,Purification and Bioactivity Characterization ofBovine Lactoferricin Peptide in Escherichia coli
Author(s):
Liu Kehang1; Zong Xicui2; Zhang Huidan1; Wanyan Yangke1; Chen Yuqing1
(1.School of Life Sciences,Nanjing Normal University,Institute of Biochemistry and Biological Products,Nanjing 210023,China)(2.Department of Basic Western Medicine,Nanjing University of Chinese Medicine Hanlin College,Taizhou 225300,China)
Keywords:
Bovine lactoferricin; antimicrobial peptide; tandem expression; SUMO
PACS:
Q291
DOI:
10.3969/j.issn.1001-4616.2020.02.016
Abstract:
Bovine lactoferricin(LfcinB)is a cationic antimicrobial peptides with activities of anti-bacteria and anti-tumor. Here it was reported that a method of producing tandem multimers of LfcinB using SUMO(small ubiquitin-related modifier)tag in Escherichia coli. Results showed that the expression level of the soluble fusion protein with SUMO tag was more than 90% of total target protein,and the dimer of LfcinB was expressed at a higher level than monomer and trimer when fused with SUMO tag. After using SUMO-specific protease to remove the SUMO tag,hydroxylamine to release monomer,the purified LfcinB was obtained with a yield of approximately 15 mg/L. The biological assays demonstrated that the recombinant LfcinB exhibited a certain antimicrobial and antitumor activity. Altogether,the SUMO fusion system with tandem repeat expression technology provides a potential production method for functional antimicrobial peptides.

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Last Update: 2020-05-15