|Table of Contents|

Loss of tom70(Translocase of Outer Mitochondrial 70)Results in Changes of Cell Dynamics in Fission Yeast(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2020年03期
Page:
120-128
Research Field:
·生物学·
Publishing date:

Info

Title:
Loss of tom70(Translocase of Outer Mitochondrial 70)Results in Changes of Cell Dynamics in Fission Yeast
Author(s):
Yuan Rongmei1; Ding Xiang2; Tan Xiumei1; Hou Yiling1
(1.College of Life Sciences,China West Normal University,Key Laboratory of Southwest China Wildlife ResourcesConservation(Ministry of Education),Nanchong 637009,China)(2.College of Environmental Science and Engineering,China West Normal University,Nanchong 637009,China)
Keywords:
tom70; mitosis; cell cycle; cell dynamics; microtubule
PACS:
Q934
DOI:
10.3969/j.issn.1001-4616.2020.03.019
Abstract:
The tom70 encodes the tom70 receptor of the complex of translocase of outer mitochondrial,which plays an important role in the transfer of mitochondrial protein into the mitochondrial membrane space. Schizosaccharomyces pombe was used as material to study the dynamic changes of cell mitosis after tom70 gene deletion. The results showed that the number and length of microtubules were abnormal in the interphase,and the length of microtubules was longer in the tom70Δ cells than in the wild type. At the same time,the formation,growth rate,growth time,length and fracture mode of the spindle were different from those in the wild type. The statistics of spindle length showed that there was a phenomenon of delayed spindle rupture in the tom70Δ cells. Three kinds of spindle fracture behaviors were observed,linear,arched and S-shaped. The results showed that the deletion of mitochondrial tom70 gene led to the defects of cell dynamics,including spindle maintenance,chromosome separation and spindle breakage.

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Last Update: 2020-09-15