|Table of Contents|

Development of SNP Markers for Pomacea canaliculata andApplication of Species Identification in P.maculata(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2021年03期
Page:
96-102
Research Field:
·生物学·
Publishing date:

Info

Title:
Development of SNP Markers for Pomacea canaliculata andApplication of Species Identification in P.maculata
Author(s):
Lin Ying1; Xiao Qi1; Lin Youfu2; Li Xuexia2; Yin Wenli2; Li Hong1; Chen Lian2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)
Keywords:
Pomacea canaliculata; P.maculata; whole genome resequencing; SNPs; molecular identification
PACS:
K826.15
DOI:
10.3969/j.issn.1001-4616.2021.03.015
Abstract:
Pomacea canaliculata is one of the 100 world’s worst invasive alien species,which causes serious economic and environmental damage in the invaded areas. Both P.canaliculata and P.maculata invaded most southern provinces in China. In order to investigate the population genetic structure and diversity of these taxa,22 single nucleotide polymorphism markers(SNP)were isolated from the whole genome sequencing data of Pomacea spp.. Polymorphisms of these SNPs were uated in P.canaliculata from Lishui City,Zhejiang Province. The results showed that the observed,expected heterozygosity and polymorphism information content per locus ranged from 0 to 0.933,0.033 to 0.549,0.032 to 0.466,respectively. Five SNPs were identified with the high Fst values and different allele frequency between P.canaliculata and P.maculata. Then these five diagnostic SNPs were genotyping in 30 samples of P.canaliculata and 18 samples of P.maculata. Species identification accuracy of single SNP locus was high(>84.21%)and the accuracy could reach 100% when three to five diagnostic SNPs were used simultaneously. Those polymorphic SNP markers will be helpful for population genetics study on P.canaliculata and efficient and accurate species identification of P.canaliculata and P.maculata.

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Last Update: 2021-09-15