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Research on TLR8 Gene Immune Response Function of BottlenoseDolphins(Tursiops truncatus)and Cattle(Bos taurus)(PDF)

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

Issue:
2021年04期
Page:
43-52
Research Field:
·生物学·
Publishing date:

Info

Title:
Research on TLR8 Gene Immune Response Function of BottlenoseDolphins(Tursiops truncatus)and Cattle(Bos taurus)
Author(s):
Gu Long; Zhang Weijing; Yu Fangfang; Ren Wenhua
School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
Keywords:
Tursiops truncatus; Bos taurus; TLR8; NF-κB; IL-8
PACS:
Q594
DOI:
10.3969/j.issn.1001-4616.2021.04.006
Abstract:
Cetaceans are the descendants of terrestrial mammals. They transitioned from land to ocean about 56 million years ago. The autoimmune function of cetaceans evolved to meet the challenges of marine pathogens. TLR8 gene is a member of the viral Toll-like receptor family. It activates downstream signal pathways after being induced by pathogens,and plays an important role in regulating the body’s immunity against pathogen infection. In this study,we analyzed the structure of the TLR8 gene of the bottlenose dolphin(Tursiops truncatus)and cattle(Bos taurus),cloned the TLR8 gene and constructed a recombinant plasmid to transfect the human embryonic kidney cell line HEK293 cells,and then stimulated the TLR8 synthetic agonist R848 Cells,to detect the differences in the expression of NF-κB and IL-8 downstream of the TLR8 signaling pathway. The experimental results showed that the expression levels of NF-κB and IL-8 of the two species were significantly higher than those of the empty vector transfection group,while the expression levels of bottlenose dolphins were lower than those of cattle. It is speculated that the TLR8 gene exerts an immune function in response to heterologous stimuli. In order to adapt to the marine environment different from the terrestrial environment,cetaceans have adopted different coping strategies and models from terrestrial animals.

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Last Update: 2021-12-15